Development of amperometric biosensors for the determination of glycolic acid in real samples
| dc.contributor.author | Tsiafoulis, C. G. | en |
| dc.contributor.author | Prodromidis, M. I. | en |
| dc.contributor.author | Karayannis, M. I. | en |
| dc.date.accessioned | 2015-11-24T16:56:01Z | |
| dc.date.available | 2015-11-24T16:56:01Z | |
| dc.identifier.issn | 0003-2700 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10381 | |
| dc.rights | Default Licence | - |
| dc.subject | performance liquid-chromatography | en |
| dc.subject | alpha-hydroxy acids | en |
| dc.subject | glyoxylic-acid | en |
| dc.subject | carboxylic-acids | en |
| dc.subject | oxidase | en |
| dc.subject | extraction | en |
| dc.subject | electrode | en |
| dc.subject | tissue | en |
| dc.subject | urine | en |
| dc.title | Development of amperometric biosensors for the determination of glycolic acid in real samples | en |
| heal.abstract | The first enzyme-based biosensors capable of determining glycolic acid in various complex matrixes, such as cosmetics, instant coffee, and urine, are presented in this paper. Two separate designs, both based on a three-membrane configuration consisting of an inner cellulose acetate membrane (CA) and an outer polycarbonate membrane (PC), which sandwich a membrane bearing the biomolecule(s), are proposed. Glycolate oxidase was immobilized onto a modified polyethersulfonate membrane by means of chemical bonding, and glycolate oxidase/catalase enzyme mixture was immobilized into a mixed-ester cellulose acetate membrane through physical adsorption. The membrane assemblies were mounted on an amperometric flow cell (hydrogen peroxide detection at a platinum anode poised at +0.65 V vs Ag/AgCl/3 KCl) or on an oxygen electrode, respectively. Both configurations were optimized with respect to various working parameters. The proposed biosensors are interference-free to common electroactive species and were successfully applied for the determination of glycolic acid in various samples, showing an excellent agreement with a reference photometric method. The validity of the proposed method in samples, in which the reference method was not applicable, was tested with recovery studies. Values of 102 +/- % were obtained. Inherent interference of oxalic acid was manipulated by using a primary amine-containing buffer and the enzyme catalase. Both systems were designed in order to be compatible with the current technology of the most widely used commercial analyzers. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1021/Ac0106896 | - |
| heal.identifier.secondary | <Go to ISI>://000173086200026 | - |
| heal.identifier.secondary | http://pubs.acs.org/doi/pdfplus/10.1021/ac0106896 | - |
| heal.journalName | Anal Chem | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2002 | - |
| heal.publisher | American Chemical Society | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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