Modulation of elongation factor-1 delta (EF-1 delta) expression by oncogenes in human epithelial cells

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dc.contributor.authorKolettas, E.en
dc.contributor.authorLymboura, M.en
dc.contributor.authorKhazaie, K.en
dc.contributor.authorLuqmani, Y.en
dc.date.accessioned2015-11-24T19:04:09Z
dc.date.available2015-11-24T19:04:09Z
dc.identifier.issn0250-7005-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19982
dc.rightsDefault Licence-
dc.subjectCloning, Molecularen
dc.subjectDNA, Neoplasm/geneticsen
dc.subjectDexamethasone/pharmacologyen
dc.subjectEpithelial Cells/metabolismen
dc.subjectGene Expression Regulation, Neoplasticen
dc.subjectGenes, rasen
dc.subjectGlucocorticoids/pharmacologyen
dc.subjectHumansen
dc.subjectOncogene Protein p21(ras)/geneticsen
dc.subjectOncogenesen
dc.subjectPeptide Elongation Factor 1en
dc.subjectPeptide Elongation Factors/*metabolismen
dc.subjectRNA, Messenger/geneticsen
dc.subjectReceptor, Epidermal Growth Factor/geneticsen
dc.subjectReceptor, erbB-2/geneticsen
dc.subjectSignal Transductionen
dc.subjectTransduction, Geneticen
dc.subjectTumor Cells, Cultureden
dc.titleModulation of elongation factor-1 delta (EF-1 delta) expression by oncogenes in human epithelial cellsen
heal.abstractA cDNA clone covering part of the C-terminal domain of human EF-1 delta was isolated from mammary cancer cells by subtractive hybridisation. The higher expression of EF-1 delta in the tumours suggested that malignant transformation in vivo requires an increase in translation factor mRNA and protein synthesis for entry into and transition through the cell cycle. To explore the relation between cell division and EF-1 delta expression, MCF-7 cells were treated with dexamethasone, an inducer of differentiation. There was no change in the mRNA levels of EF-1 delta in the dexamethasone-treated cells. To explore the relation between oncogenes and EF-1 delta expression, a variety of oncogenes were introduced into human mammary epithelial cells (MCF-7) and human keratinocytes (HaCaT). Despite high oncogene mRNA expression, there was no significant change in the EF-1 delta mRNA level by v-src, c-erbB (EGF Receptor), c-erbB-2, v-myc and v-fos oncogenes. However, overexpression of v-Ha-ras in HaCaT cells resulted in a three to five-fold decrease in the steady-state mRNA level of EF-1 delta. Taken together, the data provides further support on the interaction of translation factors and oncogenic transformation.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9568107-
heal.journalNameAnticancer Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1998-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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