Engineering lipid vesicles of enhanced intratumoral transport capabilities: correlating liposome characteristics with penetration into human prostate tumor spheroids

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dc.contributor.authorKostarelos, K.en
dc.contributor.authorEmfietzoglou, D.en
dc.contributor.authorPapakostas, A.en
dc.contributor.authorYang, W. H.en
dc.contributor.authorBallangrud, A.en
dc.contributor.authorSgouros, G.en
dc.date.accessioned2015-11-24T19:19:17Z
dc.date.available2015-11-24T19:19:17Z
dc.identifier.issn0898-2104-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21949
dc.rightsDefault Licence-
dc.subjectHumansen
dc.subjectLipid Bilayers/chemistry/metabolismen
dc.subject*Liposomes/chemistry/metabolism/therapeutic useen
dc.subjectMaleen
dc.subjectMicroscopy, Electronen
dc.subjectPhospholipids/chemistryen
dc.subjectProstatic Neoplasms/*drug therapy/*metabolismen
dc.subject*Spheroids, Cellularen
dc.subjectTumor Cells, Cultureden
dc.titleEngineering lipid vesicles of enhanced intratumoral transport capabilities: correlating liposome characteristics with penetration into human prostate tumor spheroidsen
heal.abstractLiposomes have been widely used delivery systems, particularly relevant to the development of cancer therapeutics. Numerous liposome-based drugs are in the clinic or in clinical trials today against multiple tumor types; however, systematic studies of liposome interactions with solid or metastatic tumor nodules are scarce. This study is describing the in vitro interaction between liposomes and avascular human prostate (LNCaP-LN3) tumor spheroids. The ability of fluorescently labelled liposomal delivery systems of varying physicochemical characteristics to penetrate within multicellular tumor spheroids has been investigated by confocal laser scanning microscopy. A variety of liposome characteristics and experimental parameters were investigated, including lipid bilayer composition, duration of liposome-spheroid interaction, mean liposome size, steric stabilization of liposomes. Electrostatic binding between cationic liposomes and spheroids was very efficient; however, it impeded any significant penetration of the vesicles within deeper layers of the tumor spheroid. Small unilamellar liposomes of neutral surface character did not bind as efficiently but exhibited enhanced penetrative transport capabilities closer to the tumor core. Polymer-coated (sterically stabilised) liposomes exhibited almost no interaction with the spheroid, indicating that their limited diffusion within avascular tissues may be a limiting step for their use against micrometastases. Multicellular tumor spheroids were used as models of solid tumor interstitium relevant to delivery systems able to extravasate from the microcapillaries or as models of prevascularized micrometastases. This study illustrates that interactions between liposomes and other drug delivery systems with multicellular tumor spheroids can offer critically important information with respect to optimizing solid or micrometastatic tumor delivery and targeting strategies.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1081/LPR-64953-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/16194925-
heal.identifier.secondaryhttp://informahealthcare.com/doi/abs/10.1081/LPR-64953-
heal.journalNameJ Liposome Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2005-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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