Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections

dc.contributor.authorPapadimitriou, C. S.en
dc.contributor.authorStephanaki-Nikou, S. N.en
dc.contributor.authorMalamou-Mitsi, V. D.en
dc.date.accessioned2015-11-24T19:28:49Z
dc.date.available2015-11-24T19:28:49Z
dc.identifier.issn0340-6075-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22929
dc.rightsDefault Licence-
dc.subjectChymotrypsin/analysis/antagonists & inhibitorsen
dc.subjectFibronectins/analysisen
dc.subjectHistiocytes/chemistryen
dc.subjectHistocytochemistryen
dc.subjectHumansen
dc.subjectImmunoenzyme Techniquesen
dc.subjectImmunoglobulins/analysisen
dc.subjectLymph Nodes/*pathologyen
dc.subjectLymphadenitis/*pathologyen
dc.subjectMast Cells/chemistryen
dc.subjectMonocytes/chemistryen
dc.subjectMuramidase/analysisen
dc.subjectPlasma Cells/chemistryen
dc.subjectalpha 1-Antichymotrypsinen
dc.subjectalpha 1-Antitrypsin/analysisen
dc.titleComparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sectionsen
heal.abstractThe immunoperoxidase technique after trypsinization was used on paraffin sections of 24 lymph nodes with reactive lymphadenitis and abundant nests of T-associated plasma cells. These cells were negative for all the markers investigated, which were: intracytoplasmic immunoglobulins (CIg), a1-antichymotrypsin(a1-ACT), a1-antitrypsin(a1-AT), lysozyme(Lz) and fibronectin. Other categories of cells were positive or negative depending on their type. The best markers for polymorphs proved to be a1-AT and Lz, and for monocytes and histiocytes a1-ACT and Lz. Sinus histiocytes in particular were much more constantly and strongly positive for a1-ACT than for Lz. Endothelial cells appeared almost always positive for a1-ACT and were also occasionally positive for a1-AT. Fibronectin was consistently positive in mast cells and sometimes positive in other cells, especially those of monohistiocytic origin. Our present findings are against a B-cell or monohistiocytic origin for T-associated plasma cells.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/6136117-
heal.journalNameVirchows Arch B Cell Pathol Incl Mol Patholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1983-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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