A lamin B receptor in the nuclear envelope
| dc.contributor.author | Worman, H. J. | en |
| dc.contributor.author | Yuan, J. | en |
| dc.contributor.author | Blobel, G. | en |
| dc.contributor.author | Georgatos, S. D. | en |
| dc.date.accessioned | 2015-11-24T19:40:20Z | |
| dc.date.available | 2015-11-24T19:40:20Z | |
| dc.identifier.issn | 0027-8424 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/24336 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Cell Membrane/metabolism | en |
| dc.subject | Erythrocytes/metabolism | en |
| dc.subject | Fluorescent Antibody Technique | en |
| dc.subject | Kinetics | en |
| dc.subject | Lamin Type A | en |
| dc.subject | Lamin Type B | en |
| dc.subject | Lamins | en |
| dc.subject | Membrane Proteins/*metabolism | en |
| dc.subject | Nuclear Envelope/*metabolism | en |
| dc.subject | Nuclear Proteins/*blood | en |
| dc.subject | Receptors, Cell Surface/*metabolism | en |
| dc.subject | *Receptors, Cytoplasmic and Nuclear | en |
| dc.subject | Turkeys | en |
| dc.title | A lamin B receptor in the nuclear envelope | en |
| heal.abstract | Using a solution binding assay, we show that purified 125I-labeled lamin B binds in a saturable and specific fashion to lamin-depleted avian erythrocyte nuclear membranes with a Kd of approximately 0.2 microM. This binding is significantly greater than the binding of 125I-labeled lamin A and is competitively inhibited by unlabeled ligand. We demonstrate that a 58-kDa integral membrane protein (p58) is a lamin B receptor by virtue of its abundance in the nuclear envelope and association with 125I-labeled lamin B in ligand blotting assays. Specific antibodies raised against p58 recognize one protein in isolated nuclei and partially block 125I-labeled lamin B binding to lamin-depleted nuclear membranes. Cell fractionation and indirect immunofluorescence microscopy show that p58 is located in the periphery of the nucleus. This protein may serve as a membrane attachment site for the nuclear lamina by acting as a specific receptor for lamin B. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/2847165 | - |
| heal.identifier.secondary | http://www.pnas.org/content/85/22/8531.full.pdf | - |
| heal.journalName | Proc Natl Acad Sci U S A | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1988 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
Αρχεία
Πρωτότυπος φάκελος/πακέτο
1 - 1 of 1
Φόρτωση...
- Ονομα:
- Worman-1988-A lamin B receptor i.pdf
- Μέγεθος:
- 1.08 MB
- Μορφότυπο:
- Adobe Portable Document Format
Φάκελος/Πακέτο αδειών
1 - 1 of 1
Φόρτωση...
- Ονομα:
- license.txt
- Μέγεθος:
- 1.74 KB
- Μορφότυπο:
- Item-specific license agreed upon to submission
- Περιγραφή: