Evaluation of five methods for determining low-density lipoprotein cholesterol (LDL-C) in hemodialysis patients
Φόρτωση...
Ημερομηνία
Συγγραφείς
Bairaktari, E.
Elisaf, M.
Tzallas, C.
Karabina, S. A.
Tselepis, A. D.
Siamopoulos, K. C.
Tsolas, O.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Clin Biochem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Objectives: Current recommendations for the management of dyslipidemia are largely based on the concentration of LDL-C. Most clinical laboratories estimate the concentration of LDL-C by the recommended routine method, the equation of Friedewald, in specimens from fasting subjects and with TG concentrations < 4.52 mmol/L. Because of the limitations of the Friedewald calculation, direct methods for an accurate quantification of LDL-C are needed. Design and Methods: In the present study we evaluated the accuracy of the following 5 different procedures for LDL-C in 98 patients on hemodialysis: the Friedewald equation, where LDL-C is calculated from HDL-C, measured either by the precipitation procedure with dextran sulfate-Mg2+ (Method 1), or by a direct HDL-C assay (Method 2), the Direct LDLTM assay (Method 3), the homogeneous N-geneous(TM) LDL assay (Method 4) and the calculated LDL-C values deriving from the ApoB based equation: 0.41TC - 0.32TG + 1.70ApoB - 0.27, (Clin Chem 1997;43:808-815) (Method 5). Results: All five LDL-C methods were found to be in good agreement with ultracentrifugation/dextran sulfate-Mg2+ precipitation with the coefficients of correlation of the assays to ranging between 0.93-0.95. However, significant differences in the mean values and biases vs. the reference method were observed. The Friedewald equation and the Direct assay were less affected by high LDfrC levels, and they presented higher sensitivity and higher negative predictive value. The N-geneous assay and the ApoB derived calculation were less affected by high triglyceride levels, and they presented higher specificity and higher positive predictive value. At the diagnostic LDL-C level of 3.37 mmol/L, both Friedewald calculations correctly classified 82/92 patients; Direct assay 86/98; N-geneous assay 88198; and ApoB derived calculation 88/98. At the diagnostic LDL-C level of 2.98 mmol/L., Friedewald calculations (Method 1 and Method 2) correctly classified 82/92 and 81/92 patients, respectively; Direct assay (LDL-3) 87/98; N-geneous assay (LDL-4) 91/98; and ApoB derived calculation (LDL-5) 91/98. Conclusions: Among hemodialysis patients, who commonly present "average" LDL-C concentrations and high TG levels, the N-geneous assay and the apoB derived calculation seem to yield more acceptable results for the estimation of LDL-C. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
method comparison, triglycerides, hemodialysis patients, chronic-renal-failure, dextran sulfate-mg2+ method, hdl-cholesterol, lipid abnormalities, homogeneous assay, cardiovascular-disease, analytical performance, multicenter evaluation, pediatric population, clinical-performance
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000174033100001
http://ac.els-cdn.com/S0009912001002740/1-s2.0-S0009912001002740-main.pdf?_tid=35577f0458a5409459842e241953832e&acdnat=1333110944_40e5aabe0419f6f5e75b37516c782ba1
http://ac.els-cdn.com/S0009912001002740/1-s2.0-S0009912001002740-main.pdf?_tid=35577f0458a5409459842e241953832e&acdnat=1333110944_40e5aabe0419f6f5e75b37516c782ba1
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας