Expression of immunomodulatory genes in human monocytes induced by voriconazole in the presence of Aspergillus fumigatus

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dc.contributor.authorSimitsopoulou, M.en
dc.contributor.authorRoilides, E.en
dc.contributor.authorLikartsis, C.en
dc.contributor.authorIoannidis, J.en
dc.contributor.authorOrfanou, A.en
dc.contributor.authorPaliogianni, F.en
dc.contributor.authorWalsh, T. J.en
dc.date.accessioned2015-11-24T19:16:39Z
dc.date.available2015-11-24T19:16:39Z
dc.identifier.issn0066-4804-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21693
dc.rightsDefault Licence-
dc.subjectAntifungal Agents/*pharmacologyen
dc.subjectAspergillus fumigatus/*immunologyen
dc.subjectCells, Cultureden
dc.subjectChemokine CCL2/biosynthesisen
dc.subjectChemokine CCL3en
dc.subjectChemokine CCL4en
dc.subjectDNA, Complementary/biosynthesis/geneticsen
dc.subjectGene Expression Regulation/*drug effectsen
dc.subjectHumansen
dc.subjectHyphae/immunologyen
dc.subjectImmunity, Innate/*drug effects/*geneticsen
dc.subjectInterleukin-10/biosynthesisen
dc.subjectInterleukin-12/biosynthesisen
dc.subjectInterleukin-1beta/biosynthesisen
dc.subjectMacrophage Inflammatory Proteins/biosynthesisen
dc.subjectMonocytes/drug effects/*immunologyen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectPyrimidines/*pharmacologyen
dc.subjectRNA/biosynthesis/geneticsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectTriazoles/*pharmacologyen
dc.subjectTumor Necrosis Factor-alpha/biosynthesisen
dc.titleExpression of immunomodulatory genes in human monocytes induced by voriconazole in the presence of Aspergillus fumigatusen
heal.abstractWe assessed the effect of voriconazole (VRC) on the expression and release of selected cytokines and chemokines in the THP-1 human monocytic cell line in response to Aspergillus fumigatus hyphal fragments (HF) by cDNA microarray analysis, reverse transcriptase (RT) PCR, and enzyme-linked immunosorbent assay. Stimulation of THP-1 cells by HF alone caused a significant up-regulation of CCL4 (MIP1B) and CCL16, while CCL2 (MCP1) was down-regulated. By comparison, in the presence of VRC, a large number of genes such as CCL3 (MIP1A), CCL4 (MIP1B), CCL5 (RANTES), CCL7 (MCP3), CCL11 (EOTAXIN), CCL15 (MIP1Delta), CXCL6, and CXCL13 were strongly up-regulated in THP-1 cells challenged by HF, whereas CCL20 (MIP3A) and CCL21 (MIP2) were down-regulated. Among five genes differentially expressed in THP-1 cells, IL12A, IL12B, and IL-16 were down-regulated whereas IL-11 and TGFB1 were significantly up-regulated in the presence of VRC. The inflammation-related genes IFNgamma, IL1R1, and TNFA were also up-regulated in THP-1 cells exposed to HF only in the presence of VRC. RT-PCR of four selected genes validated the results of microarrays. The release of interleukin 1beta (IL-1beta) and IL-12 was significantly increased from monocytes stimulated either by HF alone (P < 0.05) or in the presence of VRC (P < 0.01 and P < 0.05, respectively). In contrast, tumor necrosis factor alpha release from monocytes was enhanced only in the presence of VRC (P < 0.01). The chemokines monocyte chemoattractant protein 1 and macrophage inflammatory protein 1beta were decreased under both conditions (P < 0.01). These results demonstrate that in the presence of VRC, HF induces a more pronounced profile of gene expression in THP-1 cells than HF alone, potentially leading to more-efficient host resistance to A. fumigatus.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1128/AAC.01095-06-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/17178797-
heal.identifier.secondaryhttp://aac.asm.org/content/51/3/1048.full.pdf-
heal.journalNameAntimicrob Agents Chemotheren
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2007-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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