Differential pulse polarography: a method for the direct study of biosorption of metal ions by live bacteria from mixed metal solutions
| dc.contributor.author | Savvaidis, I. | en |
| dc.contributor.author | Hughes, M. N. | en |
| dc.contributor.author | Poole, R. K. | en |
| dc.date.accessioned | 2015-11-24T16:56:14Z | |
| dc.date.available | 2015-11-24T16:56:14Z | |
| dc.identifier.issn | 0003-6072 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10405 | |
| dc.rights | Default Licence | - |
| dc.subject | binary metal mixtures | en |
| dc.subject | bioaccumulation | en |
| dc.subject | metal-microbe interactions | en |
| dc.subject | methods | en |
| dc.subject | pseudomonas sp. | en |
| dc.subject | voltammetry | en |
| dc.subject | escherichia-coli | en |
| dc.subject | binding | en |
| dc.subject | cadmium | en |
| dc.subject | biomass | en |
| dc.subject | copper | en |
| dc.title | Differential pulse polarography: a method for the direct study of biosorption of metal ions by live bacteria from mixed metal solutions | en |
| heal.abstract | The technique of differential pulse polarography is shown here to be applicable to the monitoring directly the biosorption of metal ions from solution by live bacteria from mixed metal solutions. Biosorption of Cd(II), Zn(II) and Ni(II) by P. cepacia was followed using data obtained at the potential which is characteristic of the metal ion in the absence and presence of cells. Hepes buffer ( pH 7.4, 50 mM) was used as a supporting electrolyte in the polarographic chamber and metal ion peaks in the presence of cells of lower amplitude were obtained due to metal-binding by the cells. Well defined polarographic peaks were obtained in experiments involving mixtures of metal ions of Cd(II)-Zn(II), Cu(II)-Zn(II), Cu(II)-Cd(II) and Cd(II)-Ni(II). Biosorption of Cd(II), Zn(II) increased with solution pH. The method was also tested as a rapid technique for assessing removal of metal ions by live bacteria and the ability of the polarographic technique in measuring biosorption of metal ions from mixed metal solutions is demonstrated. Cu(II) was preferentially bound and removal of metals was in the order Cu(II). Ni(II) > Zn(II), Cd(II) by intact cells of P. cepacia. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1023/A:1025489915704 | - |
| heal.identifier.secondary | <Go to ISI>://000184988500003 | - |
| heal.identifier.secondary | http://www.springerlink.com/content/m04h850424k7m730/fulltext.pdf | - |
| heal.journalName | Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2003 | - |
| heal.publisher | Kluwer Academic Publishers | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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