Identification of a tripartite basal promoter which regulates human terminal deoxynucleotidyl transferase gene expression

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dc.contributor.authorBhaumik, D.en
dc.contributor.authorYang, B.en
dc.contributor.authorTrangas, T.en
dc.contributor.authorBartlett, J. S.en
dc.contributor.authorColeman, M. S.en
dc.contributor.authorSorscher, D. H.en
dc.date.accessioned2015-11-24T16:35:05Z
dc.date.available2015-11-24T16:35:05Z
dc.identifier.issn0021-9258-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7896
dc.rightsDefault Licence-
dc.subjectBase Sequenceen
dc.subjectCell Lineen
dc.subjectDNA Nucleotidylexotransferase/*biosynthesis/*geneticsen
dc.subjectDNA Primersen
dc.subjectExonsen
dc.subjectGene Expressionen
dc.subject*Gene Expression Regulation, Enzymologicen
dc.subjectHumansen
dc.subjectLeukemiaen
dc.subjectLymphomaen
dc.subjectMolecular Sequence Dataen
dc.subjectPolymerase Chain Reactionen
dc.subject*Promoter Regen
dc.titleIdentification of a tripartite basal promoter which regulates human terminal deoxynucleotidyl transferase gene expressionen
heal.abstractIn order to locate the promoter region of the human terminal deoxynucleotidyl transferase gene, serially truncated segments of the 5'-flanking region of the gene were cloned into a chloramphenicol acetyltransferase reporter vector. Transient transfection analyses of the terminal transferase-reporter gene constructs identified the basal promoter region within -34 to +40 base pairs relative to the transcription start site. Three promoter elements were defined in this region. The primary element is within 34 base pairs upstream of the transcription start site. The CAP site is 62 base pairs upstream of the translation start site. The secondary element involves sequences around the transcription start site. The third is located 25 base pairs downstream from the initiation site (+25 to +40). This tripartite basal promoter was not tissue specific; similar patterns of promoter activity were observed in terminal transferase expressing and non-expressing cells. Transfection analyses also indicated the presence of negative regulatory elements upstream of the basal promoter region, and these elements were preferentially active in cells expressing terminal transferase.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8195241-
heal.journalNameJournal of Biological Chemistryen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1994-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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