Species-independent expression of nitric oxide synthase in the sarcolemma region of visceral and somatic striated muscle fibers
Φόρτωση...
Ημερομηνία
Συγγραφείς
Grozdanovic, Z.
Nakos, G.
Dahrmann, G.
Mayer, B.
Gossrau, R.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Cell Tissue Res
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The expression and distribution of nitric oxide synthase (NOS) was studied by use of the newly designed specific histochemical NADPH diaphorase staining method and the indirect immunofluorescence technique employing an antiserum to brain NOS in visceral and somatic striated muscles of several mammalian species. Histochemical activity and immunoreactivity were located in the sarcolemma region of type I and II fibers of all muscles investigated. Visceral muscles were more strongly stained than somatic muscles. Furthermore, type II fibers, identified by staining of myosin adenosine triphosphatase activity after pre-incubation at alkaline pH, were more intensely labeled than type I fibers. In addition, NOS activity was detected in the area of the sarcolemma of intrafusal fibers. No obvious differences between species were observed. It was concluded that NOS of striated muscles probably makes up the richest and most important nitric oxide source in mammals.
Περιγραφή
Λέξεις-κλειδιά
Animals, Callithrix, Cricetinae, Female, Fluorescent Antibody Technique, Gerbillinae, Guinea Pigs, Male, Mice, Mice, Inbred Strains, Muscle Fibers, Skeletal/cytology/*enzymology, Muscle, Skeletal/cytology/enzymology, Muscle, Smooth/cytology/enzymology, Myosins/analysis, NADPH Dehydrogenase/analysis, Nitric Oxide Synthase/analysis/*biosynthesis, Organ Specificity, Rats, Rats, Wistar, Sarcolemma/*enzymology/ultrastructure, Species Specificity
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/7553769
http://www.springerlink.com/content/k16316x638u2k670/fulltext.pdf
http://www.springerlink.com/content/k16316x638u2k670/fulltext.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής