Species-independent expression of nitric oxide synthase in the sarcolemma region of visceral and somatic striated muscle fibers

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dc.contributor.authorGrozdanovic, Z.en
dc.contributor.authorNakos, G.en
dc.contributor.authorDahrmann, G.en
dc.contributor.authorMayer, B.en
dc.contributor.authorGossrau, R.en
dc.date.accessioned2015-11-24T18:50:56Z
dc.date.available2015-11-24T18:50:56Z
dc.identifier.issn0302-766X-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18180
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectCallithrixen
dc.subjectCricetinaeen
dc.subjectFemaleen
dc.subjectFluorescent Antibody Techniqueen
dc.subjectGerbillinaeen
dc.subjectGuinea Pigsen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred Strainsen
dc.subjectMuscle Fibers, Skeletal/cytology/*enzymologyen
dc.subjectMuscle, Skeletal/cytology/enzymologyen
dc.subjectMuscle, Smooth/cytology/enzymologyen
dc.subjectMyosins/analysisen
dc.subjectNADPH Dehydrogenase/analysisen
dc.subjectNitric Oxide Synthase/analysis/*biosynthesisen
dc.subjectOrgan Specificityen
dc.subjectRatsen
dc.subjectRats, Wistaren
dc.subjectSarcolemma/*enzymology/ultrastructureen
dc.subjectSpecies Specificityen
dc.titleSpecies-independent expression of nitric oxide synthase in the sarcolemma region of visceral and somatic striated muscle fibersen
heal.abstractThe expression and distribution of nitric oxide synthase (NOS) was studied by use of the newly designed specific histochemical NADPH diaphorase staining method and the indirect immunofluorescence technique employing an antiserum to brain NOS in visceral and somatic striated muscles of several mammalian species. Histochemical activity and immunoreactivity were located in the sarcolemma region of type I and II fibers of all muscles investigated. Visceral muscles were more strongly stained than somatic muscles. Furthermore, type II fibers, identified by staining of myosin adenosine triphosphatase activity after pre-incubation at alkaline pH, were more intensely labeled than type I fibers. In addition, NOS activity was detected in the area of the sarcolemma of intrafusal fibers. No obvious differences between species were observed. It was concluded that NOS of striated muscles probably makes up the richest and most important nitric oxide source in mammals.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/7553769-
heal.identifier.secondaryhttp://www.springerlink.com/content/k16316x638u2k670/fulltext.pdf-
heal.journalNameCell Tissue Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1995-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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