Improvements in qualitative characteristics of cryopreserved human spermatozoa following recovery via the SpermPrep II filtration method
Φόρτωση...
Ημερομηνία
Συγγραφείς
Zavos, P. M.
Sofikitis, N.
Toda, T.
Miyagawa, I.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Tohoku J Exp Med
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Motile, morphologically normal human spermatozoa can be separated from semen via a disposable SpermPrep filtration method. This method was employed with great success using frozen-thawed spermatozoa. The present study examined the qualitative characteristics of filtered spermatozoa via a new SpermPrep II filtration method in conjunction with the short-term freeze preservation of these spermatozoa. The isolation procedure yielded populations of spermatozoa with very high percentage motility and progressive motility grade (0-4) and which were free of seminal debris. For 20 semen samples the prefreeze values of percentage of motility and grade for the fresh and post-filtered were 58.6% and 3.1 vs 84.1% and 3.6, respectively. The sperm freeze preservation procedure involved dilution of the spermatozoa in Test-Yolk buffer (TYB) with 7% glycerol (v/v) and freezing and thawing in a conventional manner. Post-thaw percent motility, and survival % were substantially higher (p less than 0.05) for the filtered fraction than for the parent semen. The filtered fractions yielded post-thaw mean values of motility percent and grade of 68.9 and 3.4 vs 47.6 and 3.0 for the parent semen, respectively. Survival following incubation at 37 degrees C for 3 hours yielded respective values of 75.9% and 53.2% (p less than 0.05). It was concluded that the major factor in improving post-thaw quality recovery overall was the filtration via the SpermPrep II as applied in this study. This technique could have significant clinical applications in the use of frozen-thawed specimens for non-coital reproduction purposes.
Περιγραφή
Λέξεις-κλειδιά
Cryopreservation/*methods, Filtration/*methods, Humans, Male, Semen Preservation/*methods, Sperm Motility/*physiology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/1818436
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής