Improvements in qualitative characteristics of cryopreserved human spermatozoa following recovery via the SpermPrep II filtration method
| dc.contributor.author | Zavos, P. M. | en |
| dc.contributor.author | Sofikitis, N. | en |
| dc.contributor.author | Toda, T. | en |
| dc.contributor.author | Miyagawa, I. | en |
| dc.date.accessioned | 2015-11-24T19:11:21Z | |
| dc.date.available | 2015-11-24T19:11:21Z | |
| dc.identifier.issn | 0040-8727 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/20922 | |
| dc.rights | Default Licence | - |
| dc.subject | Cryopreservation/*methods | en |
| dc.subject | Filtration/*methods | en |
| dc.subject | Humans | en |
| dc.subject | Male | en |
| dc.subject | Semen Preservation/*methods | en |
| dc.subject | Sperm Motility/*physiology | en |
| dc.title | Improvements in qualitative characteristics of cryopreserved human spermatozoa following recovery via the SpermPrep II filtration method | en |
| heal.abstract | Motile, morphologically normal human spermatozoa can be separated from semen via a disposable SpermPrep filtration method. This method was employed with great success using frozen-thawed spermatozoa. The present study examined the qualitative characteristics of filtered spermatozoa via a new SpermPrep II filtration method in conjunction with the short-term freeze preservation of these spermatozoa. The isolation procedure yielded populations of spermatozoa with very high percentage motility and progressive motility grade (0-4) and which were free of seminal debris. For 20 semen samples the prefreeze values of percentage of motility and grade for the fresh and post-filtered were 58.6% and 3.1 vs 84.1% and 3.6, respectively. The sperm freeze preservation procedure involved dilution of the spermatozoa in Test-Yolk buffer (TYB) with 7% glycerol (v/v) and freezing and thawing in a conventional manner. Post-thaw percent motility, and survival % were substantially higher (p less than 0.05) for the filtered fraction than for the parent semen. The filtered fractions yielded post-thaw mean values of motility percent and grade of 68.9 and 3.4 vs 47.6 and 3.0 for the parent semen, respectively. Survival following incubation at 37 degrees C for 3 hours yielded respective values of 75.9% and 53.2% (p less than 0.05). It was concluded that the major factor in improving post-thaw quality recovery overall was the filtration via the SpermPrep II as applied in this study. This technique could have significant clinical applications in the use of frozen-thawed specimens for non-coital reproduction purposes. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/1818436 | - |
| heal.journalName | Tohoku J Exp Med | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1991 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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