Development of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella typhimurium in pure cultures of type strains and inoculated real samples
Φόρτωση...
Ημερομηνία
Συγγραφείς
Pournaras, A. V.
Koraki, T.
Prodromidis, M. I.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Analytica Chimica Acta
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The development of a faradic impedimetric immurrosensor based on electropolymerized polytyramine (Ptyr) films for the detection of S. typhimurium in milk is described for the first time. Polyclonal anti-Salmonella was cross-linked, in the presence of glutaraldehyde vapors, on Ptyr-modified gold electrodes. The dielectric behaviour of Ptyr films was evaluated with capacitance measurements, while their stability in neutral aqueous solutions was examined with impedimetric measurements. The effect of the concentration of tyramine in the forming solution on both the sensitivity and the dynamic range of the resulted immunosensors was also investigated. The alteration of the interfacial features of the electrodes due to different modification or recognition steps, was measured by faradic electrochemical impedance spectroscopy in the presence of a hexacyanoferrate(II)/(III) redox couple. At samples containing a low initial concentration of 10 cfu mL(-1) S. typhimurium, that actually defines the LOD of the immunosensors, signal changes of 33% and 88% were achieved after 3 and 10h incubation, respectively. To achieve the working simplicity expected by a biosensor, immunoreaction was performed directly in cultures. This resulted in the elimination of various centrifugation and washing steps, which are used for the isolation of bacteria cells from the culture, thus making the proposed immunosensors promising candidates for on-site applications. Finally, the proposed immunosensors were successfully used for the detection of S. typhimurium in experimentally inoculated milk samples. (c) 2008 Published by Elsevier B.V.
Περιγραφή
Λέξεις-κλειδιά
faradic electrochemical impedance spectroscopy, immunosensors, salmonella typhimurium, electropolymerized polytyramine films, on-site detection of pathogens, electrodeposited polytyramine, impedance spectroscopy, immobilization matrix, enzyme immobilization, rapid detection, biosensors, pcr, immunoassay, capacitance, resistance
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000259112800016
http://ac.els-cdn.com/S0003267008012762/1-s2.0-S0003267008012762-main.pdf?_tid=e8dbad8a8e1ddfccd4278ca2d70faa9a&acdnat=1333037250_780c9ef39c86de896f37557ef46d7a70
http://ac.els-cdn.com/S0003267008012762/1-s2.0-S0003267008012762-main.pdf?_tid=e8dbad8a8e1ddfccd4278ca2d70faa9a&acdnat=1333037250_780c9ef39c86de896f37557ef46d7a70
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας