Development of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella typhimurium in pure cultures of type strains and inoculated real samples

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dc.contributor.authorPournaras, A. V.en
dc.contributor.authorKoraki, T.en
dc.contributor.authorProdromidis, M. I.en
dc.date.accessioned2015-11-24T16:56:03Z
dc.date.available2015-11-24T16:56:03Z
dc.identifier.issn0003-2670-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10384
dc.rightsDefault Licence-
dc.subjectfaradic electrochemical impedance spectroscopyen
dc.subjectimmunosensorsen
dc.subjectsalmonella typhimuriumen
dc.subjectelectropolymerized polytyramine filmsen
dc.subjecton-site detection of pathogensen
dc.subjectelectrodeposited polytyramineen
dc.subjectimpedance spectroscopyen
dc.subjectimmobilization matrixen
dc.subjectenzyme immobilizationen
dc.subjectrapid detectionen
dc.subjectbiosensorsen
dc.subjectpcren
dc.subjectimmunoassayen
dc.subjectcapacitanceen
dc.subjectresistanceen
dc.titleDevelopment of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella typhimurium in pure cultures of type strains and inoculated real samplesen
heal.abstractThe development of a faradic impedimetric immurrosensor based on electropolymerized polytyramine (Ptyr) films for the detection of S. typhimurium in milk is described for the first time. Polyclonal anti-Salmonella was cross-linked, in the presence of glutaraldehyde vapors, on Ptyr-modified gold electrodes. The dielectric behaviour of Ptyr films was evaluated with capacitance measurements, while their stability in neutral aqueous solutions was examined with impedimetric measurements. The effect of the concentration of tyramine in the forming solution on both the sensitivity and the dynamic range of the resulted immunosensors was also investigated. The alteration of the interfacial features of the electrodes due to different modification or recognition steps, was measured by faradic electrochemical impedance spectroscopy in the presence of a hexacyanoferrate(II)/(III) redox couple. At samples containing a low initial concentration of 10 cfu mL(-1) S. typhimurium, that actually defines the LOD of the immunosensors, signal changes of 33% and 88% were achieved after 3 and 10h incubation, respectively. To achieve the working simplicity expected by a biosensor, immunoreaction was performed directly in cultures. This resulted in the elimination of various centrifugation and washing steps, which are used for the isolation of bacteria cells from the culture, thus making the proposed immunosensors promising candidates for on-site applications. Finally, the proposed immunosensors were successfully used for the detection of S. typhimurium in experimentally inoculated milk samples. (c) 2008 Published by Elsevier B.V.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1016/j.aca.2008.06.043-
heal.identifier.secondary<Go to ISI>://000259112800016-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0003267008012762/1-s2.0-S0003267008012762-main.pdf?_tid=e8dbad8a8e1ddfccd4278ca2d70faa9a&acdnat=1333037250_780c9ef39c86de896f37557ef46d7a70-
heal.journalNameAnalytica Chimica Actaen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2008-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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