Acute lung injury in a rat model of intestinal ischemia-reperfusion: The potential time depended role of phospholipases A(2)
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kostopanagiotou, G.
Avgerinos, E.
Costopanagiotou, C.
Arkadopoulos, N.
Andreadou, I.
Diamantopoulou, K.
Lekka, M.
Smyrniotis, V.
Nakos, G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Surgical Research
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Background. A pivotal role of phospholipase A (PLA(2)) and platelet-activating factor-acetylhydrolase (PAF-AcH) as enzymes involved in lung inflammation has recently been suggested. The objective of this study was to elucidate the role and the time dependence of PLA(2) and PAF-AcH fluctuations in the lung relative to the evolution of intestinal ischemia-reperfusion (IIR). Materials and methods. Rats were randomly allocated to five groups of IIR induced by occlusion of the superior mesenteric artery for 45 min followed by 1 min, 2, 4, and 8 h of reperfusion (expGroups) and five corresponding sham groups (sGroups). Bronchoalveolar lavage fluid was obtained from the right lung and its biochemical (protein, PLA(2), PAF-AcH) and cytological characteristics were determined. Plasma malonyldialdehyde was measured as a marker of lipid peroxidation. The 4 and 8 h reperfusion expGroups had significantly (P < 0.05) elevated alveolar-arterial O-2 gradient values compared with the corresponding controls. Total protein, PLA2 and PAF-AcH levels significantly (P < 0.05) increased in expGroups compared with the corresponding shams after 4 h of reperfusion. Total bronchoalveolar lavage fluid cells and plasma malonyldialdehyde were significantly (P < 0.05) elevated in expGroups compared with the sGroups after 2 h of reperfusion. Conclusions. PIA could act synergistically or parallel with the reactive oxygen species produced during IIR, resulting in the induction or even in the exacerbation of the inflammatory reaction in acute respiratory distress syndrome. PAF-AcH could play an anti-inflammatory role by reducing the concentration of PAF. (C) 2008 Elsevier Inc. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
intestinal ischemia-reperfusion, acute respiratory distress syndrome, acute lung injury, phospholipase a(2), platelet-activating factor, platelet-activating factor acetylhydrolase, platelet-activating-factor, respiratory-distress-syndrome, bronchoalveolar lavage fluid, factor acetylhydrolase, clinical-trial, organ failure, severe sepsis, paf, multicenter, inhibition
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000255668500016
http://ac.els-cdn.com/S0022480407004842/1-s2.0-S0022480407004842-main.pdf?_tid=4183b8fc5cce4ceebc14667d60a2de7c&acdnat=1333033713_0deeed6f5a06b8c44fac572c1c816d69
http://ac.els-cdn.com/S0022480407004842/1-s2.0-S0022480407004842-main.pdf?_tid=4183b8fc5cce4ceebc14667d60a2de7c&acdnat=1333033713_0deeed6f5a06b8c44fac572c1c816d69
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας