Nuclear localization of human AP endonuclease 1 (HAP1/Ref-1) associates with prognosis in early operable non-small cell lung cancer (NSCLC)
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kakolyris, S.
Giatromanolaki, A.
Koukourakis, M.
Kaklamanis, L.
Kanavaros, P.
Hickson, I. D.
Barzilay, G.
Georgoulias, V.
Gatter, K. C.
Harris, A. L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Pathol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The present study examined the immunohistochemical expression of human AP endonuclease 1 (HAP1/Ref-1), the major endonuclease in the repair of apurinic/apyrimidinic (AP) sites in cellular DNA, in normal lung and lung carcinomas. Cellular expression of HAP1 was determined using a standard avidin-biotin-peroxidase complex (ABC) technique and an anti-HAP1 rabbit polyclonal antibody on paraffin-embedded tissue sections from normal lung and in 103 primary non-small cell lung carcinomas (NSCLCs). In normal lung, the staining for HAP1 was found to be both nuclear and cytoplasmic in the pneumocytes of the alveoli. Superficial ciliated cells of the bronchial epithelium presented cytoplasmic staining, while staining for the basal cells was mostly nuclear. Bronchial glandular cells demonstrated mixed nuclear and cytoplasmic staining. Lung carcinomas showed all patterns of expression for HAP1. Loss of HAP1 expression was associated with low proliferation index (p=0.01) and with squamous histology (p=0.04). In squamous carcinomas, a significant correlation was observed between positive nuclear HAP1 and negative p53 expression (p=0.03). A survival benefit was seen in patients presenting nuclear HAP1 expression and those presenting the nuclear HAP1+/p53- phenotype (p=0.01 and 0.007, respectively). It is concluded that nuclear HAP1 localization may be relevant to its role as a DNA repair protein and/or to the recently proposed role as an activator of wild-type p53, and thus to the better outcome seen in this group of patients.
Περιγραφή
Λέξεις-κλειδιά
Aged, Carbon-Oxygen Lyases/*metabolism, Carcinoma, Non-Small-Cell Lung/*enzymology/pathology, Carcinoma, Squamous Cell/enzymology/pathology, Cell Nucleus/enzymology, DNA-(Apurinic or Apyrimidinic Site) Lyase, Deoxyribonuclease IV (Phage T4-Induced), Female, Humans, Immunoenzyme Techniques, Lung/enzymology, Lung Neoplasms/*enzymology/pathology, Male, Middle Aged, Neoplasm Proteins/*metabolism, Prognosis, Survival Rate, Tumor Suppressor Protein p53/metabolism
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/10547596
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1096-9896(199911)189:3<351::AID-PATH435>3.0.CO;2-1/asset/435_ftp.pdf?v=1&t=h0ezazwg&s=ffe938a67774bebe897fe6b3df2ff9a40dd91498
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1096-9896(199911)189:3<351::AID-PATH435>3.0.CO;2-1/asset/435_ftp.pdf?v=1&t=h0ezazwg&s=ffe938a67774bebe897fe6b3df2ff9a40dd91498
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής