Nuclear localization of human AP endonuclease 1 (HAP1/Ref-1) associates with prognosis in early operable non-small cell lung cancer (NSCLC)

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dc.contributor.authorKakolyris, S.en
dc.contributor.authorGiatromanolaki, A.en
dc.contributor.authorKoukourakis, M.en
dc.contributor.authorKaklamanis, L.en
dc.contributor.authorKanavaros, P.en
dc.contributor.authorHickson, I. D.en
dc.contributor.authorBarzilay, G.en
dc.contributor.authorGeorgoulias, V.en
dc.contributor.authorGatter, K. C.en
dc.contributor.authorHarris, A. L.en
dc.date.accessioned2015-11-24T19:01:23Z
dc.date.available2015-11-24T19:01:23Z
dc.identifier.issn0022-3417-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19682
dc.rightsDefault Licence-
dc.subjectAgeden
dc.subjectCarbon-Oxygen Lyases/*metabolismen
dc.subjectCarcinoma, Non-Small-Cell Lung/*enzymology/pathologyen
dc.subjectCarcinoma, Squamous Cell/enzymology/pathologyen
dc.subjectCell Nucleus/enzymologyen
dc.subjectDNA-(Apurinic or Apyrimidinic Site) Lyaseen
dc.subjectDeoxyribonuclease IV (Phage T4-Induced)en
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectImmunoenzyme Techniquesen
dc.subjectLung/enzymologyen
dc.subjectLung Neoplasms/*enzymology/pathologyen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectNeoplasm Proteins/*metabolismen
dc.subjectPrognosisen
dc.subjectSurvival Rateen
dc.subjectTumor Suppressor Protein p53/metabolismen
dc.titleNuclear localization of human AP endonuclease 1 (HAP1/Ref-1) associates with prognosis in early operable non-small cell lung cancer (NSCLC)en
heal.abstractThe present study examined the immunohistochemical expression of human AP endonuclease 1 (HAP1/Ref-1), the major endonuclease in the repair of apurinic/apyrimidinic (AP) sites in cellular DNA, in normal lung and lung carcinomas. Cellular expression of HAP1 was determined using a standard avidin-biotin-peroxidase complex (ABC) technique and an anti-HAP1 rabbit polyclonal antibody on paraffin-embedded tissue sections from normal lung and in 103 primary non-small cell lung carcinomas (NSCLCs). In normal lung, the staining for HAP1 was found to be both nuclear and cytoplasmic in the pneumocytes of the alveoli. Superficial ciliated cells of the bronchial epithelium presented cytoplasmic staining, while staining for the basal cells was mostly nuclear. Bronchial glandular cells demonstrated mixed nuclear and cytoplasmic staining. Lung carcinomas showed all patterns of expression for HAP1. Loss of HAP1 expression was associated with low proliferation index (p=0.01) and with squamous histology (p=0.04). In squamous carcinomas, a significant correlation was observed between positive nuclear HAP1 and negative p53 expression (p=0.03). A survival benefit was seen in patients presenting nuclear HAP1 expression and those presenting the nuclear HAP1+/p53- phenotype (p=0.01 and 0.007, respectively). It is concluded that nuclear HAP1 localization may be relevant to its role as a DNA repair protein and/or to the recently proposed role as an activator of wild-type p53, and thus to the better outcome seen in this group of patients.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1002/(SICI)1096-9896(199911)189:3<351::AID-PATH435>3.0.CO;2-1-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10547596-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/(SICI)1096-9896(199911)189:3<351::AID-PATH435>3.0.CO;2-1/asset/435_ftp.pdf?v=1&t=h0ezazwg&s=ffe938a67774bebe897fe6b3df2ff9a40dd91498-
heal.journalNameJ Patholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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