Quantifiable mRNA transcripts for tamoxifen-metabolising enzymes in human endometrium
Φόρτωση...
Ημερομηνία
Συγγραφείς
Singh, M. N.
Stringfellow, H. F.
Walsh, M. J.
Ashton, K. M.
Paraskevaidis, E.
Abdo, K. R.
Martin-Hirsch, P. L.
Phillips, D. H.
Martin, F. L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Toxicology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Tamoxifen has been used in the management of receptor-positive breast cancer for >20 years. Usage confers an elevated risk of developing endometrial carcinoma. Its mechanism of carcinogenicity remains unresolved with controversy as to whether or not this is mediated through a genotoxic mechanism. Usage is not only associated with an elevated occurrence of endometrioid endometrial carcinoma, but also type 2 and mixed epithelial-stromal tumours (MESTs) that have a poorer prognosis. Following hysterectomy, endometrial tissues (n=18) classified as benign (n=6), non-tamoxifen-associated carcinoma (n=6) and tamoxifen-associated carcinoma (n=6) were obtained; quantitative gene expression was performed. Employing real-time RT-PCR, the relative gene expressions of phase I/II metabolic enzymes CYP1A2, CYP1B1 and CYP3A4, cathechol-O-methyltransferase (COMT) and SULT2A1 were ascertained. Measurable mRNA transcripts, especially for those genes associated with tamoxifen bioactivation, were quantifiable in all the tissues examined. Whether this is evidence that generation of genotoxic tamoxifen metabolites may occur in human endometrial tissue remains to be ascertained.
Περιγραφή
Λέξεις-κλειδιά
Adult, Aged, Aged, 80 and over, Antineoplastic Agents, Hormonal/*pharmacokinetics, Biotransformation, Carcinoma/*enzymology/genetics, Cytochrome P-450 Enzyme System/*genetics/metabolism, Endometrial Neoplasms/*enzymology/genetics, Endometrium/*enzymology, Female, Gene Expression Regulation, Neoplastic, Humans, Middle Aged, RNA, Messenger/*metabolism, Sulfotransferases/genetics/metabolism, Tamoxifen/*pharmacokinetics
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/18502016
http://ac.els-cdn.com/S0300483X08001704/1-s2.0-S0300483X08001704-main.pdf?_tid=0f201c7a8c02cb9dc58c1760399d8881&acdnat=1333714046_38606db497d40458e951c7c2a0c7c45c
http://ac.els-cdn.com/S0300483X08001704/1-s2.0-S0300483X08001704-main.pdf?_tid=0f201c7a8c02cb9dc58c1760399d8881&acdnat=1333714046_38606db497d40458e951c7c2a0c7c45c
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής