Quantifiable mRNA transcripts for tamoxifen-metabolising enzymes in human endometrium

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dc.contributor.authorSingh, M. N.en
dc.contributor.authorStringfellow, H. F.en
dc.contributor.authorWalsh, M. J.en
dc.contributor.authorAshton, K. M.en
dc.contributor.authorParaskevaidis, E.en
dc.contributor.authorAbdo, K. R.en
dc.contributor.authorMartin-Hirsch, P. L.en
dc.contributor.authorPhillips, D. H.en
dc.contributor.authorMartin, F. L.en
dc.date.accessioned2015-11-24T18:55:21Z
dc.date.available2015-11-24T18:55:21Z
dc.identifier.issn0300-483X-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18860
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectAntineoplastic Agents, Hormonal/*pharmacokineticsen
dc.subjectBiotransformationen
dc.subjectCarcinoma/*enzymology/geneticsen
dc.subjectCytochrome P-450 Enzyme System/*genetics/metabolismen
dc.subjectEndometrial Neoplasms/*enzymology/geneticsen
dc.subjectEndometrium/*enzymologyen
dc.subjectFemaleen
dc.subjectGene Expression Regulation, Neoplasticen
dc.subjectHumansen
dc.subjectMiddle Ageden
dc.subjectRNA, Messenger/*metabolismen
dc.subjectSulfotransferases/genetics/metabolismen
dc.subjectTamoxifen/*pharmacokineticsen
dc.titleQuantifiable mRNA transcripts for tamoxifen-metabolising enzymes in human endometriumen
heal.abstractTamoxifen has been used in the management of receptor-positive breast cancer for >20 years. Usage confers an elevated risk of developing endometrial carcinoma. Its mechanism of carcinogenicity remains unresolved with controversy as to whether or not this is mediated through a genotoxic mechanism. Usage is not only associated with an elevated occurrence of endometrioid endometrial carcinoma, but also type 2 and mixed epithelial-stromal tumours (MESTs) that have a poorer prognosis. Following hysterectomy, endometrial tissues (n=18) classified as benign (n=6), non-tamoxifen-associated carcinoma (n=6) and tamoxifen-associated carcinoma (n=6) were obtained; quantitative gene expression was performed. Employing real-time RT-PCR, the relative gene expressions of phase I/II metabolic enzymes CYP1A2, CYP1B1 and CYP3A4, cathechol-O-methyltransferase (COMT) and SULT2A1 were ascertained. Measurable mRNA transcripts, especially for those genes associated with tamoxifen bioactivation, were quantifiable in all the tissues examined. Whether this is evidence that generation of genotoxic tamoxifen metabolites may occur in human endometrial tissue remains to be ascertained.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1016/j.tox.2008.04.009-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/18502016-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0300483X08001704/1-s2.0-S0300483X08001704-main.pdf?_tid=0f201c7a8c02cb9dc58c1760399d8881&acdnat=1333714046_38606db497d40458e951c7c2a0c7c45c-
heal.journalNameToxicologyen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2008-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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