A palmitoyl-tailed sequential oligopeptide carrier for engineering immunogenic conjugates
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kargakis, M.
Zevgiti, S.
Krikorian, D.
Sakarellos-Daitsiotis, M.
Sakarellos, C.
Panou-Pornonis, E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Vaccine
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The main guideline in designing effective immunogens as vaccine candidates capable of eliciting potent and specific immune responses is to combine B/T cell epitopes and adjuvants as immunostimulators on the same carrier that links the major histocompatibility complex with T cell receptors. Aiming at contributing to the development of carriers for human usage a helicoid type sequential oligopeptide carrier, SOCn-II, formed by the repeating tetrapeptide unit (Aib-Lys-Aib-Gly)(n), n = 2-7, elongated from the amino-terminus by the palmitoyl group, known for its adjuvanticity, is now presented. The main B cell epitope, PPGMRPP, of the Sm autoantigen against which the majority of antibodies in patients with systemic lupus erythematosus is directed, was coupled to the Lys-(NH2)-H-epsilon groups of the carrier in four copies and the resulting conjugate Palm-SOC4-II-Sm-4 was subjected to animal immunizations without utilizing any adjuvant. The induced immune response was comparable with that produced when Ac-SOC4-II-Sm-4 was administered in animals following the conventional immunization protocol of complete/incomplete Freund's adjuvant. High titers of anti-Palm-SOC4-II-Sm-4 antibodies were generated, which recognize the priming immunogenic conjugate, as well as reconstituted Sm mimics but not the carrier alone. It is concluded that Palm-SOCn-II carrier is a valuable tool for engineering immunogens eliciting enhanced and specific humoral immune responses.
Περιγραφή
Λέξεις-κλειδιά
sequential oligopeptide carriers, immunogenic conjugates, palmitoyl immunostimulator, antibody recognition, sm autoantigen, epitope, peptides, socn, determinants, conformation, antigens
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000249850300007
http://ac.els-cdn.com/S0264410X07007827/1-s2.0-S0264410X07007827-main.pdf?_tid=ddede9e1c6fa969eb80f47554d86acb3&acdnat=1333038504_ea8d63a5a152b752f3acdd9ca7fa9bf4
http://ac.els-cdn.com/S0264410X07007827/1-s2.0-S0264410X07007827-main.pdf?_tid=ddede9e1c6fa969eb80f47554d86acb3&acdnat=1333038504_ea8d63a5a152b752f3acdd9ca7fa9bf4
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας