A palmitoyl-tailed sequential oligopeptide carrier for engineering immunogenic conjugates
| dc.contributor.author | Kargakis, M. | en |
| dc.contributor.author | Zevgiti, S. | en |
| dc.contributor.author | Krikorian, D. | en |
| dc.contributor.author | Sakarellos-Daitsiotis, M. | en |
| dc.contributor.author | Sakarellos, C. | en |
| dc.contributor.author | Panou-Pornonis, E. | en |
| dc.date.accessioned | 2015-11-24T16:58:01Z | |
| dc.date.available | 2015-11-24T16:58:01Z | |
| dc.identifier.issn | 0264-410X | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10645 | |
| dc.rights | Default Licence | - |
| dc.subject | sequential oligopeptide carriers | en |
| dc.subject | immunogenic conjugates | en |
| dc.subject | palmitoyl immunostimulator | en |
| dc.subject | antibody recognition | en |
| dc.subject | sm autoantigen | en |
| dc.subject | epitope | en |
| dc.subject | peptides | en |
| dc.subject | socn | en |
| dc.subject | determinants | en |
| dc.subject | conformation | en |
| dc.subject | antigens | en |
| dc.title | A palmitoyl-tailed sequential oligopeptide carrier for engineering immunogenic conjugates | en |
| heal.abstract | The main guideline in designing effective immunogens as vaccine candidates capable of eliciting potent and specific immune responses is to combine B/T cell epitopes and adjuvants as immunostimulators on the same carrier that links the major histocompatibility complex with T cell receptors. Aiming at contributing to the development of carriers for human usage a helicoid type sequential oligopeptide carrier, SOCn-II, formed by the repeating tetrapeptide unit (Aib-Lys-Aib-Gly)(n), n = 2-7, elongated from the amino-terminus by the palmitoyl group, known for its adjuvanticity, is now presented. The main B cell epitope, PPGMRPP, of the Sm autoantigen against which the majority of antibodies in patients with systemic lupus erythematosus is directed, was coupled to the Lys-(NH2)-H-epsilon groups of the carrier in four copies and the resulting conjugate Palm-SOC4-II-Sm-4 was subjected to animal immunizations without utilizing any adjuvant. The induced immune response was comparable with that produced when Ac-SOC4-II-Sm-4 was administered in animals following the conventional immunization protocol of complete/incomplete Freund's adjuvant. High titers of anti-Palm-SOC4-II-Sm-4 antibodies were generated, which recognize the priming immunogenic conjugate, as well as reconstituted Sm mimics but not the carrier alone. It is concluded that Palm-SOCn-II carrier is a valuable tool for engineering immunogens eliciting enhanced and specific humoral immune responses. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1016/j.vaceine.2007.07.007 | - |
| heal.identifier.secondary | <Go to ISI>://000249850300007 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0264410X07007827/1-s2.0-S0264410X07007827-main.pdf?_tid=ddede9e1c6fa969eb80f47554d86acb3&acdnat=1333038504_ea8d63a5a152b752f3acdd9ca7fa9bf4 | - |
| heal.journalName | Vaccine | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2007 | - |
| heal.publisher | Elsevier | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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