Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis
Φόρτωση...
Ημερομηνία
Συγγραφείς
Topakas, E.
Vafiadi, C.
Stamatis, H.
Christakopoulos, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Enzyme and Microbial Technology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M-r 23,000 and pI 3. 1. The enzyme activity was optimal at pH 6.0 and 55 degrees C. The esterase displayed remarkable stability at pH 10.0 and retained 50% of its activity after 133 and 55 min at 55 and 60 degrees C, respectively. Determination of k(cat)/K-m revealed that the enzyme had a broad spectrum of activity against the (hydroxyl) cinnamate esters indicating a type C feruloyl esterase. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and hydrolysed 4-nitrophenyl-5-O-trans-fer-uloyl-alpha-L-arabinofuranoside three times more efficiently than 4-nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside. Ferulic acid was efficiently released from wheat bran when the esterase was incubated together with xylanase from S. thermophile (a maximum of 41% total ferulic acid released after 1 h incubation). StFacC by itself could release FA but at a level almost 10-fold lower than that obtained in the presence of xylanase. The potential of StFaeC for the synthesis of various phenolic acid esters was examined using as a reaction system a ternary water-organic mixture consisting of n-hexane, 1-butanol and water. Also StFaeC catalyzed the transfer of the feruloyl group to L-arabinose in a similar system using t-butanol, with about a 40% conversion of L-arabinose to feruloylated derivative was achieved. This work is the first example of enzymatic feruloylation of a carbohydrate. (c) 2004 Elsevier Inc. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
sporotrichum thermophile, feruloyl esterase, phenolic acid esters, transesterification, surfactantless microemulsions, fluorescens subsp cellulosa, organic-solvent mixtures, aspergillus-niger, detergentless microemulsions, substrate-specificity, catalyt
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000227781000017
http://ac.els-cdn.com/S0141022904004041/1-s2.0-S0141022904004041-main.pdf?_tid=0fb8b26ac16ea54048ac930fd9b22268&acdnat=1335510792_d40b89159ac97ecf4eda0f54231f6b41
http://ac.els-cdn.com/S0141022904004041/1-s2.0-S0141022904004041-main.pdf?_tid=0fb8b26ac16ea54048ac930fd9b22268&acdnat=1335510792_d40b89159ac97ecf4eda0f54231f6b41
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών