Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis
| dc.contributor.author | Topakas, E. | en |
| dc.contributor.author | Vafiadi, C. | en |
| dc.contributor.author | Stamatis, H. | en |
| dc.contributor.author | Christakopoulos, P. | en |
| dc.date.accessioned | 2015-11-24T16:33:37Z | |
| dc.date.available | 2015-11-24T16:33:37Z | |
| dc.identifier.issn | 0141-0229 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7686 | |
| dc.rights | Default Licence | - |
| dc.subject | sporotrichum thermophile | en |
| dc.subject | feruloyl esterase | en |
| dc.subject | phenolic acid esters | en |
| dc.subject | transesterification | en |
| dc.subject | surfactantless microemulsions | en |
| dc.subject | fluorescens subsp cellulosa | en |
| dc.subject | organic-solvent mixtures | en |
| dc.subject | aspergillus-niger | en |
| dc.subject | detergentless microemulsions | en |
| dc.subject | substrate-specificity | en |
| dc.subject | catalyt | en |
| dc.title | Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis | en |
| heal.abstract | A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M-r 23,000 and pI 3. 1. The enzyme activity was optimal at pH 6.0 and 55 degrees C. The esterase displayed remarkable stability at pH 10.0 and retained 50% of its activity after 133 and 55 min at 55 and 60 degrees C, respectively. Determination of k(cat)/K-m revealed that the enzyme had a broad spectrum of activity against the (hydroxyl) cinnamate esters indicating a type C feruloyl esterase. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and hydrolysed 4-nitrophenyl-5-O-trans-fer-uloyl-alpha-L-arabinofuranoside three times more efficiently than 4-nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside. Ferulic acid was efficiently released from wheat bran when the esterase was incubated together with xylanase from S. thermophile (a maximum of 41% total ferulic acid released after 1 h incubation). StFacC by itself could release FA but at a level almost 10-fold lower than that obtained in the presence of xylanase. The potential of StFaeC for the synthesis of various phenolic acid esters was examined using as a reaction system a ternary water-organic mixture consisting of n-hexane, 1-butanol and water. Also StFaeC catalyzed the transfer of the feruloyl group to L-arabinose in a similar system using t-butanol, with about a 40% conversion of L-arabinose to feruloylated derivative was achieved. This work is the first example of enzymatic feruloylation of a carbohydrate. (c) 2004 Elsevier Inc. All rights reserved. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1016/j.enzmictec.2004.12.020 | - |
| heal.identifier.secondary | <Go to ISI>://000227781000017 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0141022904004041/1-s2.0-S0141022904004041-main.pdf?_tid=0fb8b26ac16ea54048ac930fd9b22268&acdnat=1335510792_d40b89159ac97ecf4eda0f54231f6b41 | - |
| heal.journalName | Enzyme and Microbial Technology | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2005 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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