Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
Φόρτωση...
Ημερομηνία
Συγγραφείς
Siafakas, N.
Markoulatos, P.
Levidiotou-Stefanou, S.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Mol Cell Probes
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell cultures inoculated with treated faecal material from the patients. Serotypic identification of the isolates with mixed equine antisera pools followed and the RT-PCR amplicons were further studied by restriction fragment length polymorphism analysis and sequencing. Fifty-three clinical enterovirus strains were isolated from respective cases of suspected enterovirus infection, most of which showed the clinical symptoms of aseptic meningitis. Echovirus (ECV) 6 was the most frequently isolated serotype, followed by coxsackie B viruses, ECV13, poliovirus type 1 (PV1) vaccine strain and ECV30. Nucleotide sequence analysis showed the existence of different genetic groups on the basis of the 5'-untranslated region (5'-UTR) of the genome, which circulated in the population during the same time period. Different serotypes belonged to the same genetic group and vice versa. The 5'-UTR seems to be appropriate for the investigation of enterovirus evolution and epidemiology.
Περιγραφή
Λέξεις-κλειδιά
5' Untranslated Regions, Biological Evolution, *Disease Outbreaks, Enterovirus/classification/*isolation & purification, Enterovirus Infections/diagnosis/epidemiology, Feces/virology, Female, Greece/epidemiology, Humans, Male, Meningitis, Aseptic/epidemiology/*virology, Polymorphism, Restriction Fragment Length, RNA, Viral/*analysis, Reverse Transcriptase Polymerase Chain Reaction, Serotyping
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/15488379
http://ac.els-cdn.com/S0890850804000623/1-s2.0-S0890850804000623-main.pdf?_tid=8268c8f12c8280e79c9e07bd8b6352a7&acdnat=1332827875_050ebc2979b275ae42679a38b02e35c2
http://ac.els-cdn.com/S0890850804000623/1-s2.0-S0890850804000623-main.pdf?_tid=8268c8f12c8280e79c9e07bd8b6352a7&acdnat=1332827875_050ebc2979b275ae42679a38b02e35c2
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής