Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
| dc.contributor.author | Siafakas, N. | en |
| dc.contributor.author | Markoulatos, P. | en |
| dc.contributor.author | Levidiotou-Stefanou, S. | en |
| dc.date.accessioned | 2015-11-24T19:04:02Z | |
| dc.date.available | 2015-11-24T19:04:02Z | |
| dc.identifier.issn | 0890-8508 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/19957 | |
| dc.rights | Default Licence | - |
| dc.subject | 5' Untranslated Regions | en |
| dc.subject | Biological Evolution | en |
| dc.subject | *Disease Outbreaks | en |
| dc.subject | Enterovirus/classification/*isolation & purification | en |
| dc.subject | Enterovirus Infections/diagnosis/epidemiology | en |
| dc.subject | Feces/virology | en |
| dc.subject | Female | en |
| dc.subject | Greece/epidemiology | en |
| dc.subject | Humans | en |
| dc.subject | Male | en |
| dc.subject | Meningitis, Aseptic/epidemiology/*virology | en |
| dc.subject | Polymorphism, Restriction Fragment Length | en |
| dc.subject | RNA, Viral/*analysis | en |
| dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
| dc.subject | Serotyping | en |
| dc.title | Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology | en |
| heal.abstract | An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell cultures inoculated with treated faecal material from the patients. Serotypic identification of the isolates with mixed equine antisera pools followed and the RT-PCR amplicons were further studied by restriction fragment length polymorphism analysis and sequencing. Fifty-three clinical enterovirus strains were isolated from respective cases of suspected enterovirus infection, most of which showed the clinical symptoms of aseptic meningitis. Echovirus (ECV) 6 was the most frequently isolated serotype, followed by coxsackie B viruses, ECV13, poliovirus type 1 (PV1) vaccine strain and ECV30. Nucleotide sequence analysis showed the existence of different genetic groups on the basis of the 5'-untranslated region (5'-UTR) of the genome, which circulated in the population during the same time period. Different serotypes belonged to the same genetic group and vice versa. The 5'-UTR seems to be appropriate for the investigation of enterovirus evolution and epidemiology. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1016/j.mcp.2004.06.005 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/15488379 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0890850804000623/1-s2.0-S0890850804000623-main.pdf?_tid=8268c8f12c8280e79c9e07bd8b6352a7&acdnat=1332827875_050ebc2979b275ae42679a38b02e35c2 | - |
| heal.journalName | Mol Cell Probes | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2004 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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