Development and application of competitive ELISA assays for rat LH and FSH
Φόρτωση...
Ημερομηνία
Συγγραφείς
Pappa, A.
Seferiadis, K.
Marselos, M.
Tsolas, O.
Messinis, I. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Theriogenology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Rat LH (rLH) and FSH (rFSH) were measured by sensitive and specific competition ELISAs. The rat LH ELISA used rLH-I-9 coated plates, an antiserum against rLH and an antibody against rabbit IgG labeled with peroxidase. Using rLH-RP-3 as a standard, rat LH was determined by binding of the anti-LH antibody to rLH-I-9 coated plates. The sensitivity of the assay was 0.8 ng/mL. Similarly, the rat FSH-ELISA used rFSH-I-8 coated plates, an antiserum against rFSH and an antibody against rabbit IgG labeled with peroxidase. Using rFSH-RP-3 as a standard, the FSH-ELISA was also determined by binding of the anti-FSH antibody to rFSH-I-8 coated plates. The sensitivity of this assay was 1.25 ng/mL. Both rat LH and FSH ELISA assays are highly specific and provide accurate determination of gonadotrophins in buffers, sera, cell culture media, and anterior pituitary extracts. These assays were used for monitoring the gonadotrophin surge-attenuating factor (GnSAF) and inhibin activities present in human follicular fluid (hFF). The 2 new ELISA procedures have practical advantages (safety, convenience, economy) over the RIA methods, and they perform as well as the RIA techniques at the same range of concentrations.
Περιγραφή
Λέξεις-κλειδιά
Animals, Biological Assay, Cells, Cultured, Chromatography, Agarose, Chromatography, Gel, Enzyme-Linked Immunosorbent Assay/*methods, Female, Follicle Stimulating Hormone/*analysis/blood, Follicular Fluid/chemistry, Gonadal Hormones, Humans, Inhibins/analysis, Least-Squares Analysis, Luteinizing Hormone/*analysis/blood, Male, Pituitary Gland/chemistry/cytology, Proteins/analysis, Radioimmunoassay, Rats, Sensitivity and Specificity
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/10729014
http://ac.els-cdn.com/S0093691X99000382/1-s2.0-S0093691X99000382-main.pdf?_tid=7c0aa0d28f17c81b2a2aa67c87b20777&acdnat=1337336286_4830354459d0d89b023159341ea51fa9
http://ac.els-cdn.com/S0093691X99000382/1-s2.0-S0093691X99000382-main.pdf?_tid=7c0aa0d28f17c81b2a2aa67c87b20777&acdnat=1337336286_4830354459d0d89b023159341ea51fa9
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής