Development and application of competitive ELISA assays for rat LH and FSH

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dc.contributor.authorPappa, A.en
dc.contributor.authorSeferiadis, K.en
dc.contributor.authorMarselos, M.en
dc.contributor.authorTsolas, O.en
dc.contributor.authorMessinis, I. E.en
dc.date.accessioned2015-11-24T19:24:57Z
dc.date.available2015-11-24T19:24:57Z
dc.identifier.issn0093-691X-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22550
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBiological Assayen
dc.subjectCells, Cultureden
dc.subjectChromatography, Agaroseen
dc.subjectChromatography, Gelen
dc.subjectEnzyme-Linked Immunosorbent Assay/*methodsen
dc.subjectFemaleen
dc.subjectFollicle Stimulating Hormone/*analysis/blooden
dc.subjectFollicular Fluid/chemistryen
dc.subjectGonadal Hormonesen
dc.subjectHumansen
dc.subjectInhibins/analysisen
dc.subjectLeast-Squares Analysisen
dc.subjectLuteinizing Hormone/*analysis/blooden
dc.subjectMaleen
dc.subjectPituitary Gland/chemistry/cytologyen
dc.subjectProteins/analysisen
dc.subjectRadioimmunoassayen
dc.subjectRatsen
dc.subjectSensitivity and Specificityen
dc.titleDevelopment and application of competitive ELISA assays for rat LH and FSHen
heal.abstractRat LH (rLH) and FSH (rFSH) were measured by sensitive and specific competition ELISAs. The rat LH ELISA used rLH-I-9 coated plates, an antiserum against rLH and an antibody against rabbit IgG labeled with peroxidase. Using rLH-RP-3 as a standard, rat LH was determined by binding of the anti-LH antibody to rLH-I-9 coated plates. The sensitivity of the assay was 0.8 ng/mL. Similarly, the rat FSH-ELISA used rFSH-I-8 coated plates, an antiserum against rFSH and an antibody against rabbit IgG labeled with peroxidase. Using rFSH-RP-3 as a standard, the FSH-ELISA was also determined by binding of the anti-FSH antibody to rFSH-I-8 coated plates. The sensitivity of this assay was 1.25 ng/mL. Both rat LH and FSH ELISA assays are highly specific and provide accurate determination of gonadotrophins in buffers, sera, cell culture media, and anterior pituitary extracts. These assays were used for monitoring the gonadotrophin surge-attenuating factor (GnSAF) and inhibin activities present in human follicular fluid (hFF). The 2 new ELISA procedures have practical advantages (safety, convenience, economy) over the RIA methods, and they perform as well as the RIA techniques at the same range of concentrations.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10729014-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0093691X99000382/1-s2.0-S0093691X99000382-main.pdf?_tid=7c0aa0d28f17c81b2a2aa67c87b20777&acdnat=1337336286_4830354459d0d89b023159341ea51fa9-
heal.journalNameTheriogenologyen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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