A complementary La/SSB epitope anchored to Sequential Oligopeptide Carrier regulates the anti-La/SSB response in immunized animals
Φόρτωση...
Ημερομηνία
Συγγραφείς
Voitharou, C.
Krikorian, D.
Sakarellos, C.
Sakarellos-Daitsiotis, M.
Panou-Pomonis, E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Wiley
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Peptide Science
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Complementary peptide epitopes, derived from complementary RNA sequences, have been used for suppressing the autoimmune response in experimental autoimmune diseases as myasthenia gravis, allergic neuritis and allergic encephalomyelitis. Aiming at contributing to the development of a tool that could regulate the autoantibody production against La/SSB, which is the main target of autoantibodies in Sjogren's syndrome (SS) and systemic lupus erythematosus (SLE), the complementary epitope, cpep349-364, of the minor T/major B cell epitope of La/SSB, pep349-364, was utilized for the induction of neutralizing anti-cpep349-364 antibodies in rabbit immunizations. Complementary peptides were coupled to an artificial carrier, developed in our laboratory, in order to enhance the complementary potency of cpep349-364 and its counterpart. This carrier, named Sequential Oligopeptide Carrier, SOC(n) formed by the repeating tripeptide Lys-Aib-Gly, adopts helical conformation, which allows the anchored peptide epitopes to preserve their initial reactivity such as molecular recognition, antigenicity/immunogenicity. Our study provides proof of evidence of specific interactions between idiotypic (Id)/anti-idiotypic (ant.i-Id) antibodies generated in immunized animals by the sense epitope (conjugate 1) of La/SSB and its complementary counterpart (conjugate 11). It was also demonstrated that the Id/anti-Id association is specifically disrupted by adding either the sense epitope (conjugate 1) or its complementary counterpart (conjugate 11). A mutual neutralization of Id/anti-Id antibodies was observed in vivo, which implies that generation of anti-Id antibodies by immunization with the complementary La/SSB epitope could scavenge the anti-La/SSB response. Copyright (c) 2008 European Peptide Society and John Wiley & Sons, Ltd.
Περιγραφή
Λέξεις-κλειδιά
complementary la/ssb epitope, sequential oligopeptide carrier, regulation of anti-la/ssb response, rabbit immunizations, immunological assays, elisa inhibition assays, autoimmune myasthenia-gravis, main immunogenic region, t-cell responses, acetylcholine-receptor, b-cell, antiidiotypic antibodies, peptide vaccine, messenger-rna, encephalomyelitis, idiotype
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000259382600008
http://onlinelibrary.wiley.com/store/10.1002/psc.1043/asset/1043_ftp.pdf?v=1&t=h0e0w4jx&s=d98738ef0015c469005868065d4446a8687a6b69
http://onlinelibrary.wiley.com/store/10.1002/psc.1043/asset/1043_ftp.pdf?v=1&t=h0e0w4jx&s=d98738ef0015c469005868065d4446a8687a6b69
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας