A complementary La/SSB epitope anchored to Sequential Oligopeptide Carrier regulates the anti-La/SSB response in immunized animals

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dc.contributor.authorVoitharou, C.en
dc.contributor.authorKrikorian, D.en
dc.contributor.authorSakarellos, C.en
dc.contributor.authorSakarellos-Daitsiotis, M.en
dc.contributor.authorPanou-Pomonis, E.en
dc.date.accessioned2015-11-24T16:53:14Z
dc.date.available2015-11-24T16:53:14Z
dc.identifier.issn1075-2617-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/9990
dc.rightsDefault Licence-
dc.subjectcomplementary la/ssb epitopeen
dc.subjectsequential oligopeptide carrieren
dc.subjectregulation of anti-la/ssb responseen
dc.subjectrabbit immunizationsen
dc.subjectimmunological assaysen
dc.subjectelisa inhibition assaysen
dc.subjectautoimmune myasthenia-gravisen
dc.subjectmain immunogenic regionen
dc.subjectt-cell responsesen
dc.subjectacetylcholine-receptoren
dc.subjectb-cellen
dc.subjectantiidiotypic antibodiesen
dc.subjectpeptide vaccineen
dc.subjectmessenger-rnaen
dc.subjectencephalomyelitisen
dc.subjectidiotypeen
dc.titleA complementary La/SSB epitope anchored to Sequential Oligopeptide Carrier regulates the anti-La/SSB response in immunized animalsen
heal.abstractComplementary peptide epitopes, derived from complementary RNA sequences, have been used for suppressing the autoimmune response in experimental autoimmune diseases as myasthenia gravis, allergic neuritis and allergic encephalomyelitis. Aiming at contributing to the development of a tool that could regulate the autoantibody production against La/SSB, which is the main target of autoantibodies in Sjogren's syndrome (SS) and systemic lupus erythematosus (SLE), the complementary epitope, cpep349-364, of the minor T/major B cell epitope of La/SSB, pep349-364, was utilized for the induction of neutralizing anti-cpep349-364 antibodies in rabbit immunizations. Complementary peptides were coupled to an artificial carrier, developed in our laboratory, in order to enhance the complementary potency of cpep349-364 and its counterpart. This carrier, named Sequential Oligopeptide Carrier, SOC(n) formed by the repeating tripeptide Lys-Aib-Gly, adopts helical conformation, which allows the anchored peptide epitopes to preserve their initial reactivity such as molecular recognition, antigenicity/immunogenicity. Our study provides proof of evidence of specific interactions between idiotypic (Id)/anti-idiotypic (ant.i-Id) antibodies generated in immunized animals by the sense epitope (conjugate 1) of La/SSB and its complementary counterpart (conjugate 11). It was also demonstrated that the Id/anti-Id association is specifically disrupted by adding either the sense epitope (conjugate 1) or its complementary counterpart (conjugate 11). A mutual neutralization of Id/anti-Id antibodies was observed in vivo, which implies that generation of anti-Id antibodies by immunization with the complementary La/SSB epitope could scavenge the anti-La/SSB response. Copyright (c) 2008 European Peptide Society and John Wiley & Sons, Ltd.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1002/Psc.1043-
heal.identifier.secondary<Go to ISI>://000259382600008-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/psc.1043/asset/1043_ftp.pdf?v=1&t=h0e0w4jx&s=d98738ef0015c469005868065d4446a8687a6b69-
heal.journalNameJournal of Peptide Scienceen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2008-
heal.publisherWileyen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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