Clusterin/apolipoprotein J is a novel biomarker of cellular senescence that does not affect the proliferative capacity of human diploid fibroblasts
Φόρτωση...
Ημερομηνία
Συγγραφείς
Petropoulou, C.
Trougakos, I. P.
Kolettas, E.
Toussaint, O.
Gonos, E. S.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
FEBS Lett
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Normal human fibroblasts have a limited replicative potential in culture and eventually reach a state of irreversible growth arrest, termed senescence. In a previous study aiming to identify genes that are differentially regulated during cellular senescence we have cloned clusterin/apolipoprotein J (Apo J), a 80 kDa secreted glycoprotein. In the current report we pursue our studies and show that senescence of human diploid fibroblasts is accompanied by up-regulation of both Apo J mRNA and protein levels, but with no altered biogenesis, binding partner profile or intracellular distribution of the two Apo J forms detected. To analyze the causal relationship between senescence and Apo J protein accumulation, we stably overexpressed the Apo J gene in primary as well as in SV40 T antigen-immortalized human fibroblasts and we showed no alteration of the proliferative capacity of the transduced cells. Despite previous reports on tumor-derived cell lines, overexpression of Apo J in human fibroblasts did not provide protection against apoptosis or growth arrest induced by hydrogen peroxide. Overall, our results suggest that Apo J overexpression does not induce senescence but it is rather a secondary consequence of the senescence phenotype. To our knowledge this is the first report that provides a functional analysis of human Apo J during replicative senescence.
Περιγραφή
Λέξεις-κλειδιά
Antigens, Differentiation/biosynthesis/genetics/*isolation & purification, Cell Aging/*physiology, Clusterin, Diploidy, Fibroblasts/cytology/*physiology, Glycoproteins/biosynthesis/genetics/*isolation & purification, Humans, Molecular Chaperones/biosynthesis/genetics/*isolation & purification, Oxidative Stress/physiology, Recombinant Proteins/biosynthesis, Up-Regulation
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/11741605
http://ac.els-cdn.com/S0014579301031507/1-s2.0-S0014579301031507-main.pdf?_tid=b8d9bc05985860b1f09f7f2a6d2f15ec&acdnat=1333000694_553717e9eac488f82619d39f0617c32e
http://ac.els-cdn.com/S0014579301031507/1-s2.0-S0014579301031507-main.pdf?_tid=b8d9bc05985860b1f09f7f2a6d2f15ec&acdnat=1333000694_553717e9eac488f82619d39f0617c32e
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής