Clusterin/apolipoprotein J is a novel biomarker of cellular senescence that does not affect the proliferative capacity of human diploid fibroblasts
| dc.contributor.author | Petropoulou, C. | en |
| dc.contributor.author | Trougakos, I. P. | en |
| dc.contributor.author | Kolettas, E. | en |
| dc.contributor.author | Toussaint, O. | en |
| dc.contributor.author | Gonos, E. S. | en |
| dc.date.accessioned | 2015-11-24T19:30:10Z | |
| dc.date.available | 2015-11-24T19:30:10Z | |
| dc.identifier.issn | 0014-5793 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23032 | |
| dc.rights | Default Licence | - |
| dc.subject | Antigens, Differentiation/biosynthesis/genetics/*isolation & purification | en |
| dc.subject | Cell Aging/*physiology | en |
| dc.subject | Clusterin | en |
| dc.subject | Diploidy | en |
| dc.subject | Fibroblasts/cytology/*physiology | en |
| dc.subject | Glycoproteins/biosynthesis/genetics/*isolation & purification | en |
| dc.subject | Humans | en |
| dc.subject | Molecular Chaperones/biosynthesis/genetics/*isolation & purification | en |
| dc.subject | Oxidative Stress/physiology | en |
| dc.subject | Recombinant Proteins/biosynthesis | en |
| dc.subject | Up-Regulation | en |
| dc.title | Clusterin/apolipoprotein J is a novel biomarker of cellular senescence that does not affect the proliferative capacity of human diploid fibroblasts | en |
| heal.abstract | Normal human fibroblasts have a limited replicative potential in culture and eventually reach a state of irreversible growth arrest, termed senescence. In a previous study aiming to identify genes that are differentially regulated during cellular senescence we have cloned clusterin/apolipoprotein J (Apo J), a 80 kDa secreted glycoprotein. In the current report we pursue our studies and show that senescence of human diploid fibroblasts is accompanied by up-regulation of both Apo J mRNA and protein levels, but with no altered biogenesis, binding partner profile or intracellular distribution of the two Apo J forms detected. To analyze the causal relationship between senescence and Apo J protein accumulation, we stably overexpressed the Apo J gene in primary as well as in SV40 T antigen-immortalized human fibroblasts and we showed no alteration of the proliferative capacity of the transduced cells. Despite previous reports on tumor-derived cell lines, overexpression of Apo J in human fibroblasts did not provide protection against apoptosis or growth arrest induced by hydrogen peroxide. Overall, our results suggest that Apo J overexpression does not induce senescence but it is rather a secondary consequence of the senescence phenotype. To our knowledge this is the first report that provides a functional analysis of human Apo J during replicative senescence. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11741605 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0014579301031507/1-s2.0-S0014579301031507-main.pdf?_tid=b8d9bc05985860b1f09f7f2a6d2f15ec&acdnat=1333000694_553717e9eac488f82619d39f0617c32e | - |
| heal.journalName | FEBS Lett | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2001 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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