Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases

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dc.contributor.authorChristakopoulos, P.en
dc.contributor.authorKatapodis, P.en
dc.contributor.authorKalogeris, E.en
dc.contributor.authorKekos, D.en
dc.contributor.authorMacris, B. J.en
dc.contributor.authorStamatis, H.en
dc.contributor.authorSkaltsa, H.en
dc.date.accessioned2015-11-24T16:32:56Z
dc.date.available2015-11-24T16:32:56Z
dc.identifier.issn0141-8130-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7599
dc.rightsDefault Licence-
dc.subjectAnti-Bacterial Agents/*pharmacologyen
dc.subjectChromatographyen
dc.subjectChromatography, Thin Layeren
dc.subjectDose-Response Relationship, Drugen
dc.subjectEndo-1,4-beta Xylanasesen
dc.subjectHydrolysisen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectOligosaccharides/*pharmacologyen
dc.subjectUronic Acids/chemistryen
dc.subjectXylan Endo-1en
dc.titleAntimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanasesen
heal.abstractAcidic oligosaccharides were obtained from birchwood xylan by treatment with a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family 11 endoxylanases. The main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-O-methyl-D-glucuronic acid. The xylanase from T. aurantiacus liberate from glucuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from S. thermophile, which liberated an aldopentauronic acid. Acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography (SEC) and the primary structure was determined by 13C NMR spectroscopy. The acidic xylo-oligosaccharides were tested against three Gram-positive and three Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori. Aldopentauronic acid was proved more active against the Gram-positive bacteria and against H. pylori.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12568925-
heal.identifier.secondaryhttp://ac.els-cdn.com/S014181300200079X/1-s2.0-S014181300200079X-main.pdf?_tid=06687fd6-c389-11e2-92d5-00000aacb35d&acdnat=1369300586_ebe7d6754028245ec1a1b61727c8bbf1-
heal.journalNameInt J Biol Macromolen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2003-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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