Signal requirements for interleukin 4 promoter activation in human T cells
Φόρτωση...
Ημερομηνία
Συγγραφείς
Paliogianni, F.
Hama, N.
Mavrothalassitis, G. J.
Thyphronitis, G.
Boumpas, D. T.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Cell Immunol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
We have studied the signal requirements for human IL-4 promoter activation in Jurkat T cells by the use of DNA transfection assays with vectors carrying the IL-4 promoter linked to a reporter gene, Stimulation with calcium (Ca2+) ionophores (ionomycin), but not with phorbol esters (phorbol myristate acetate, PMA) or cyclic AMP elevating agents (prostaglandin Ea(2) PGE(2)), induced the transcriptional activity of the IL-4 promoter by similar to 3-fold. Costimulation with ionomycin and PGE(2) resulted in the same level of IL-4 promoter activity as the stimulation with ionomycin alone. In contrast, costimulation with ionomycin and PMA decreased the activity of the IL-4 promoter by similar to 40% compared to stimulation with ionomycin alone. Induction of IL-4 promoter by ionomycin was partially inhibited (similar to 50% inhibition) in the presence of as high as 2 mu g/ml cyclosporin A (CsA), an inhibitor of the Ca+/calmodulin-dependent phosphatase calcineurin. Under the same conditions, only 0.1 mu g/ml of CsA inhibited by >95% the transactivation of the IL-2 promoter in response to ionomycin and PMA. Transfection of a deletion mutant of the calcineurin catalytic subunit (Delta CaM-AI) known to have Ca2+-independent, constitutive phosphatase activity increased IL-4 promoter activity by similar to 14-fold. Stimulation with ionomycin of cells transfected with low doses of Delta CaM-AI, further induced IL-4 promoter activity by similar to 2-fold, These results identify the Ca2+-signaling system as a key component of the signal transduction pathway leading to IL-4 promoter activation in Jurkat T cells and suggest a major role of calcineurin in its regulation. (C) 1996 Academic Press, Inc.
Περιγραφή
Λέξεις-κλειδιά
chromosomal gene, phorbol ester, lymphocytes-t, calcineurin, transcription, il-4, camp, prostaglandin-e2, cyclosporine, t-helper-1
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1996TY85400004
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών