Signal requirements for interleukin 4 promoter activation in human T cells

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dc.contributor.authorPaliogianni, F.en
dc.contributor.authorHama, N.en
dc.contributor.authorMavrothalassitis, G. J.en
dc.contributor.authorThyphronitis, G.en
dc.contributor.authorBoumpas, D. T.en
dc.date.accessioned2015-11-24T16:31:34Z
dc.date.available2015-11-24T16:31:34Z
dc.identifier.issn0008-8749-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7460
dc.rightsDefault Licence-
dc.subjectchromosomal geneen
dc.subjectphorbol esteren
dc.subjectlymphocytes-ten
dc.subjectcalcineurinen
dc.subjecttranscriptionen
dc.subjectil-4en
dc.subjectcampen
dc.subjectprostaglandin-e2en
dc.subjectcyclosporineen
dc.subjectt-helper-1en
dc.titleSignal requirements for interleukin 4 promoter activation in human T cellsen
heal.abstractWe have studied the signal requirements for human IL-4 promoter activation in Jurkat T cells by the use of DNA transfection assays with vectors carrying the IL-4 promoter linked to a reporter gene, Stimulation with calcium (Ca2+) ionophores (ionomycin), but not with phorbol esters (phorbol myristate acetate, PMA) or cyclic AMP elevating agents (prostaglandin Ea(2) PGE(2)), induced the transcriptional activity of the IL-4 promoter by similar to 3-fold. Costimulation with ionomycin and PGE(2) resulted in the same level of IL-4 promoter activity as the stimulation with ionomycin alone. In contrast, costimulation with ionomycin and PMA decreased the activity of the IL-4 promoter by similar to 40% compared to stimulation with ionomycin alone. Induction of IL-4 promoter by ionomycin was partially inhibited (similar to 50% inhibition) in the presence of as high as 2 mu g/ml cyclosporin A (CsA), an inhibitor of the Ca+/calmodulin-dependent phosphatase calcineurin. Under the same conditions, only 0.1 mu g/ml of CsA inhibited by >95% the transactivation of the IL-2 promoter in response to ionomycin and PMA. Transfection of a deletion mutant of the calcineurin catalytic subunit (Delta CaM-AI) known to have Ca2+-independent, constitutive phosphatase activity increased IL-4 promoter activity by similar to 14-fold. Stimulation with ionomycin of cells transfected with low doses of Delta CaM-AI, further induced IL-4 promoter activity by similar to 2-fold, These results identify the Ca2+-signaling system as a key component of the signal transduction pathway leading to IL-4 promoter activation in Jurkat T cells and suggest a major role of calcineurin in its regulation. (C) 1996 Academic Press, Inc.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://A1996TY85400004-
heal.journalNameCell Immunolen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1996-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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