Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. globosa
Φόρτωση...
Ημερομηνία
Συγγραφείς
Gaitanis, G.
Velegraki, A.
Alexopoulos, E. C.
Chasapi, V.
Tsigonia, A.
Katsambas, A.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Br J Dermatol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND: The expansion of the genus Malassezia has generated interest in the epidemiological investigation of the distribution of new species in a range of dermatoses, on which variable results have been reported from different geographical regions. No data are thus far available from South-east Europe (Greece). OBJECTIVES: To study the distribution of Malassezia species in pityriasis versicolor (PV) and seborrhoeic dermatitis (SD) and to investigate whether polymorphisms in the internal transcribed spacer (ITS) 1 region facilitate detection of M. globosa and M. sympodialis subtypes. METHODS: In total, 109 patients with PV and SD and positive Malassezia cultures were included in the study. Age, gender, primary/recurrent episode, disease extent and clinical form of PV were recorded. ITS 1 polymorphisms of M. globosa and M. sympodialis type and clinical strains were investigated by polymerase chain reaction (PCR)-single-strand conformational polymorphism (SSCP) analysis. RESULTS: Malassezia globosa was the prevalent species isolated from PV and SD either alone (77% and 39%, respectively) or in combination (13% and 18%, respectively) with other Malassezia species. The pigmented form of PV was strongly correlated with the female gender. PCR-SSCP differentiated five subgroups of M. globosa with one being associated with extensive clinical disease. All M. sympodialis isolates displayed a homogeneous ITS 1 PCR-SSCP profile. CONCLUSIONS: Malassezia species isolation rates were in agreement with those reported from South-west Europe. PCR-SSCP of the ITS 1 is useful for highlighting prospective clinical implications of M. globosa subtypes.
Περιγραφή
Λέξεις-κλειδιά
Adolescent, Adult, Aged, Dermatitis, Seborrheic/*microbiology, Dermatomycoses/*microbiology, Female, Humans, Malassezia/*classification/genetics/isolation & purification, Male, Middle Aged, Mycological Typing Techniques/methods, Polymerase Chain Reaction/methods, Polymorphism, Single-Stranded Conformational, Statistics as Topic, Tinea Versicolor/*microbiology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/16634886
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2133.2005.07114.x/asset/j.1365-2133.2005.07114.x.pdf?v=1&t=h0ta0ddb&s=a5215efccd980ede5fe5544fb745e41473b79302
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2133.2005.07114.x/asset/j.1365-2133.2005.07114.x.pdf?v=1&t=h0ta0ddb&s=a5215efccd980ede5fe5544fb745e41473b79302
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής