Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. globosa

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dc.contributor.authorGaitanis, G.en
dc.contributor.authorVelegraki, A.en
dc.contributor.authorAlexopoulos, E. C.en
dc.contributor.authorChasapi, V.en
dc.contributor.authorTsigonia, A.en
dc.contributor.authorKatsambas, A.en
dc.date.accessioned2015-11-24T19:24:04Z
dc.date.available2015-11-24T19:24:04Z
dc.identifier.issn0007-0963-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22413
dc.rightsDefault Licence-
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectDermatitis, Seborrheic/*microbiologyen
dc.subjectDermatomycoses/*microbiologyen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectMalassezia/*classification/genetics/isolation & purificationen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMycological Typing Techniques/methodsen
dc.subjectPolymerase Chain Reaction/methodsen
dc.subjectPolymorphism, Single-Stranded Conformationalen
dc.subjectStatistics as Topicen
dc.subjectTinea Versicolor/*microbiologyen
dc.titleDistribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. globosaen
heal.abstractBACKGROUND: The expansion of the genus Malassezia has generated interest in the epidemiological investigation of the distribution of new species in a range of dermatoses, on which variable results have been reported from different geographical regions. No data are thus far available from South-east Europe (Greece). OBJECTIVES: To study the distribution of Malassezia species in pityriasis versicolor (PV) and seborrhoeic dermatitis (SD) and to investigate whether polymorphisms in the internal transcribed spacer (ITS) 1 region facilitate detection of M. globosa and M. sympodialis subtypes. METHODS: In total, 109 patients with PV and SD and positive Malassezia cultures were included in the study. Age, gender, primary/recurrent episode, disease extent and clinical form of PV were recorded. ITS 1 polymorphisms of M. globosa and M. sympodialis type and clinical strains were investigated by polymerase chain reaction (PCR)-single-strand conformational polymorphism (SSCP) analysis. RESULTS: Malassezia globosa was the prevalent species isolated from PV and SD either alone (77% and 39%, respectively) or in combination (13% and 18%, respectively) with other Malassezia species. The pigmented form of PV was strongly correlated with the female gender. PCR-SSCP differentiated five subgroups of M. globosa with one being associated with extensive clinical disease. All M. sympodialis isolates displayed a homogeneous ITS 1 PCR-SSCP profile. CONCLUSIONS: Malassezia species isolation rates were in agreement with those reported from South-west Europe. PCR-SSCP of the ITS 1 is useful for highlighting prospective clinical implications of M. globosa subtypes.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1111/j.1365-2133.2005.07114.x-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/16634886-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1111/j.1365-2133.2005.07114.x/asset/j.1365-2133.2005.07114.x.pdf?v=1&t=h0ta0ddb&s=a5215efccd980ede5fe5544fb745e41473b79302-
heal.journalNameBr J Dermatolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2006-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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