Amperometric determination of L-malic acid in a flow injection analysis manifold using packed-bed enzyme reactors
| dc.contributor.author | Prodromidis, M. I. | en |
| dc.contributor.author | TzouwaraKarayanni, S. M. | en |
| dc.contributor.author | Karayannis, M. I. | en |
| dc.contributor.author | Vadgama, P. | en |
| dc.contributor.author | Maines, A. | en |
| dc.date.accessioned | 2015-11-24T16:46:50Z | |
| dc.date.available | 2015-11-24T16:46:50Z | |
| dc.identifier.issn | 0003-2654 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/9111 | |
| dc.rights | Default Licence | - |
| dc.subject | amperometric malate determination | en |
| dc.subject | malate dehydrogenase and diaphorase reactors | en |
| dc.subject | flow injection analysis | en |
| dc.subject | fruits and vegetables | en |
| dc.subject | electrodes | en |
| dc.subject | lactate | en |
| dc.title | Amperometric determination of L-malic acid in a flow injection analysis manifold using packed-bed enzyme reactors | en |
| heal.abstract | An enzymic method for the determination of L-malic acid in fruits and vegetables is described, Malate dehydrogenase (MDH) and diaphorase (DI) were co-immobilized on aminopropyl-, isothiocyanate- and N-hydroxysuccinimide ester (N-HSE)-modified controlled-pore glass, which was then used for the construction of a packed-bed reactor, MDH catalyses the reaction between malate and NAD(+) and produces NADH, which converts hexacyanoferrate(III) into hexacyanoferrate(II) in the presence of the DI, Amperometric measurements with pure graphite electrodes in a fully automated flow injection manifold were used to monitor the concentration of hexacyanoferrate(II) at +0.3 V versus an Ag/AgCl reference electrode, The electrochemical behaviour of hexacyanoferrate(III), 2,6-dichlorophenolindophenol and p-benzoquinone was tested with cyclic voltammetry. Parameters such as working and immobilization pH, flow rate, sample size, temperature and NAD(+) and hexacyanoferrate(III) concentrations were investigated, The interference of various ions, amino acids and organic acids present in real samples was also studied, The calibration graph was linear for the range 2 x 10(-5)-4 x 10(-4) mol l(-1) malate, The reactors remained active for a period of more than 3 months under specified storage conditions, The maximum sample throughput was 30 h(-1) and the relative standard deviation of the method was better than 1.5% (n = 5), The mean relative error was 4% for fruits-and 2% for vegetables, The accuracy of the proposed method was tested by comparison of the results with those obtained by a reference method. Good correlation was attained, Recovery experiments showed results between 95 and 108%. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1039/AN9962100435 | - |
| heal.identifier.secondary | <Go to ISI>://A1996UG29600014 | - |
| heal.identifier.secondary | http://pubs.rsc.org/en/Content/ArticleLanding/1996/AN/an9962100435 | - |
| heal.journalName | Analyst | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1996 | - |
| heal.publisher | Royal Society of Chemistry | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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