Simultaneous determination of phenolic compounds and tocopherols in virgin olive oil using HPLC and UV detection

dc.contributor.authorTasioula-Margari, M.en
dc.contributor.authorOkogeri, O.en
dc.date.accessioned2015-11-24T16:47:21Z
dc.date.available2015-11-24T16:47:21Z
dc.identifier.issn0308-8146-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/9170
dc.rightsDefault Licence-
dc.subjectvirgin olive oilen
dc.subjectextractionen
dc.subjecthplcen
dc.subjectphenolic compoundsen
dc.subjectalpha-tocopherolen
dc.subjectperformance liquid-chromatographyen
dc.subjectlow-density-lipoproteinen
dc.subjectantioxidantsen
dc.subjectextractionen
dc.subjectoxidationen
dc.titleSimultaneous determination of phenolic compounds and tocopherols in virgin olive oil using HPLC and UV detectionen
heal.abstractA simple procedure is described for I he simultaneous extraction and HPLC determination of phenols and tocopherols in virgin olive oil. The extraction was carried out using methanol and an isopropanol- methanol mixture. Separation was achieved on a reversed phase C-18 column with acetic acid/water-methanol-acetonitrile-isopropanol mixture under gradient elution. Detection was accomplished with UV detection at lambda==280 nm. Using this method, simple and complex phenols as well as alpha -tocopherol can be determined in one run. Phenolic compounds were totally extracted whereas an average recovery of 80% was achieved for alpha -tocopherol. Validation of method is reported for olive oil samples with varying concentrations of alpha -tocopherol and phenolic compounds. (C) 2001 Elsevier Science Ltd. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1016/S0308-8146(01)00176-5-
heal.identifier.secondary<Go to ISI>://000170507600015-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0308814601001765/1-s2.0-S0308814601001765-main.pdf?_tid=e84185bc-406e-11e3-88d1-00000aab0f26&acdnat=1383033263_7891f851bd5973ba3a50e83ca3b794ce-
heal.journalNameFood Chemistryen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2001-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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