Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium
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Authors
Talbot, J. A.
Lambert, A.
Mitchell, R.
Grabinski, M.
Anderson, D. C.
Tsatsoulis, A.
Shalet, S. M.
Robertson, W. R.
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peer-reviewed
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Acta Endocrinol (Copenh)
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We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/l) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091 +/- 322 to 1480 +/- 84 pmol/l (p less than 0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360 +/- 45 to 1242 +/- 133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 mumol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 mumol/l) and A23187 (2 mumol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by greater than 80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 mumol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 mumol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.
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Keywords
Animals, Bucladesine/pharmacology, Calcimycin/pharmacology, Calcium/pharmacology, Cells, Cultured, Dose-Response Relationship, Drug, Drug Interactions, Egtazic Acid/pharmacology, Estradiol/biosynthesis, Estrogens/*secretion, Follicle Stimulating Hormone/*pharmacology, Forskolin/pharmacology, Ionomycin/pharmacology, Male, Radioimmunoassay, Rats, Rats, Inbred Strains, Sertoli Cells/*secretion
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http://www.ncbi.nlm.nih.gov/pubmed/1659097
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής