Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium

Απλή σελίδα τεκμηρίου
dc.contributor.authorTalbot, J. A.en
dc.contributor.authorLambert, A.en
dc.contributor.authorMitchell, R.en
dc.contributor.authorGrabinski, M.en
dc.contributor.authorAnderson, D. C.en
dc.contributor.authorTsatsoulis, A.en
dc.contributor.authorShalet, S. M.en
dc.contributor.authorRobertson, W. R.en
dc.date.accessioned2015-11-24T19:15:31Z
dc.date.available2015-11-24T19:15:31Z
dc.identifier.issn0001-5598-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21528
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBucladesine/pharmacologyen
dc.subjectCalcimycin/pharmacologyen
dc.subjectCalcium/pharmacologyen
dc.subjectCells, Cultureden
dc.subjectDose-Response Relationship, Drugen
dc.subjectDrug Interactionsen
dc.subjectEgtazic Acid/pharmacologyen
dc.subjectEstradiol/biosynthesisen
dc.subjectEstrogens/*secretionen
dc.subjectFollicle Stimulating Hormone/*pharmacologyen
dc.subjectForskolin/pharmacologyen
dc.subjectIonomycin/pharmacologyen
dc.subjectMaleen
dc.subjectRadioimmunoassayen
dc.subjectRatsen
dc.subjectRats, Inbred Strainsen
dc.subjectSertoli Cells/*secretionen
dc.titleFollicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calciumen
heal.abstractWe have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/l) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091 +/- 322 to 1480 +/- 84 pmol/l (p less than 0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360 +/- 45 to 1242 +/- 133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 mumol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 mumol/l) and A23187 (2 mumol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by greater than 80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 mumol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 mumol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/1659097-
heal.journalNameActa Endocrinol (Copenh)en
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1991-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ