Prothymosin alpha associates with the oncoprotein SET and is involved in chromatin decondensation
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Karetsou, Z.
Martic, G.
Tavoulari, S.
Christoforidis, S.
Wilm, M.
Gruss, C.
Papamarcaki, T.
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peer-reviewed
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FEBS Lett
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Prothymosin alpha (ProTalpha) is a histone H1-binding protein that interacts with the transcription coactivator CREB-binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two-hybrid assays, we show here that ProTalpha forms a complex with the oncoprotein SET. ProTalpha efficiently decondenses human sperm chromatin, while overexpression of GFP-ProTalpha in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTalpha function.
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Keywords
Amino Acid Sequence, Blotting, Western, CREB-Binding Protein, Cell Extracts, Chromatin/*metabolism, Chromosomal Proteins, Non-Histone, Green Fluorescent Proteins/metabolism, HeLa Cells, Histone Chaperones, Humans, Luciferases/metabolism, Male, Mass Spectrometry, Molecular Sequence Data, Molecular Weight, Nuclear Proteins/metabolism, Plasmids/metabolism, Precipitin Tests, Protein Binding, Protein Precursors/genetics/*metabolism, Proteins/chemistry/*metabolism, Recombinant Proteins/metabolism, Sequence Analysis, Protein, Silver Staining, Spermatozoa/metabolism, Thymosin/*analogs & derivatives/genetics/*metabolism, Trans-Activators/metabolism, Transcription Factors, Two-Hybrid System Techniques
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http://www.ncbi.nlm.nih.gov/pubmed/15556635
http://ac.els-cdn.com/S0014579304013092/1-s2.0-S0014579304013092-main.pdf?_tid=5f5e3605208ca5324cba8a33ba040be6&acdnat=1332915888_a34e4db20aaf673ebf5d2d976d8aed0c
http://ac.els-cdn.com/S0014579304013092/1-s2.0-S0014579304013092-main.pdf?_tid=5f5e3605208ca5324cba8a33ba040be6&acdnat=1332915888_a34e4db20aaf673ebf5d2d976d8aed0c
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής