Study of the electrochemical behavior of Disperse Blue 1-modified graphite electrodes. Application to the flow determination of NADH
Φόρτωση...
Ημερομηνία
Συγγραφείς
Stergiou, D. V.
Prodromidis, M. I.
Veltsistas, P. G.
Evmiridis, N. P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Electroanalysis
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The preparation and the electrochemical study of Disperse Blue 1-chemically modified electrodes (DB1-CME), as well as their efficiency for the electrocatalytic oxidation of NADH is described. The proposed mediator was immobilized by physical adsorption onto graphite electrodes. The electrochemical behavior of DB1-CME was studied with cyclic voltammetry. The electrochemical redox reaction of DB1 was found to be reversible, revealing two well-shaped pair of peaks with formal potentials 152 and -42 mV, respectively, (vs. Ag/AgCl/3M KCl) at pH 6.5. The current I-P has a linear relationship with the scan rate up to 800 mV s(-1), which is indicative for a fast electron transfer kinetics. The dissociation constants of the immobilized DB1 redox couple were calculated pK(1) = 4 and pK(2) = 5. The electrochemical rate constants of the immobilized DB1 were calculated k(1)degrees = 18s(-1) and k(2)degrees = 23s(-1) (Gamma = 2.36 nmol cm(-2)). The modified electrodes were mounted in a flow injection manifold, poised at + 150 mV (vs. Ag/AgCl/3M KCl) and a catalytic current due to the oxidation of NADH was measured. The reproducibility was 1.4% RSD (n = 11 for 30 muM NADH) The behavior of the sensor towards different reducing compounds was investigated. The sensor exhibited good operational and storage stability.
Περιγραφή
Λέξεις-κλειδιά
disperse blue 1-modified graphite electrode, cyclic voltammetry, electrocatalysis of nadh, flow injection analysis, dihydronicotinamide adenine-dinucleotide, chemically-modified electrodes, potential sweep voltammogram, electrocatalytic oxidation, general expression, nile blue, 2,6-dichlorophenolindophenol, stability
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000221992100011
http://onlinelibrary.wiley.com/store/10.1002/elan.200302902/asset/949_ftp.pdf?v=1&t=h1gmuys2&s=522ed2ef77dff1ec2eaadb6f125723e32125d1dd
http://onlinelibrary.wiley.com/store/10.1002/elan.200302902/asset/949_ftp.pdf?v=1&t=h1gmuys2&s=522ed2ef77dff1ec2eaadb6f125723e32125d1dd
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας