Study of the electrochemical behavior of Disperse Blue 1-modified graphite electrodes. Application to the flow determination of NADH
| dc.contributor.author | Stergiou, D. V. | en |
| dc.contributor.author | Prodromidis, M. I. | en |
| dc.contributor.author | Veltsistas, P. G. | en |
| dc.contributor.author | Evmiridis, N. P. | en |
| dc.date.accessioned | 2015-11-24T16:48:34Z | |
| dc.date.available | 2015-11-24T16:48:34Z | |
| dc.identifier.issn | 1040-0397 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/9342 | |
| dc.rights | Default Licence | - |
| dc.subject | disperse blue 1-modified graphite electrode | en |
| dc.subject | cyclic voltammetry | en |
| dc.subject | electrocatalysis of nadh | en |
| dc.subject | flow injection analysis | en |
| dc.subject | dihydronicotinamide adenine-dinucleotide | en |
| dc.subject | chemically-modified electrodes | en |
| dc.subject | potential sweep voltammogram | en |
| dc.subject | electrocatalytic oxidation | en |
| dc.subject | general expression | en |
| dc.subject | nile blue | en |
| dc.subject | 2,6-dichlorophenolindophenol | en |
| dc.subject | stability | en |
| dc.title | Study of the electrochemical behavior of Disperse Blue 1-modified graphite electrodes. Application to the flow determination of NADH | en |
| heal.abstract | The preparation and the electrochemical study of Disperse Blue 1-chemically modified electrodes (DB1-CME), as well as their efficiency for the electrocatalytic oxidation of NADH is described. The proposed mediator was immobilized by physical adsorption onto graphite electrodes. The electrochemical behavior of DB1-CME was studied with cyclic voltammetry. The electrochemical redox reaction of DB1 was found to be reversible, revealing two well-shaped pair of peaks with formal potentials 152 and -42 mV, respectively, (vs. Ag/AgCl/3M KCl) at pH 6.5. The current I-P has a linear relationship with the scan rate up to 800 mV s(-1), which is indicative for a fast electron transfer kinetics. The dissociation constants of the immobilized DB1 redox couple were calculated pK(1) = 4 and pK(2) = 5. The electrochemical rate constants of the immobilized DB1 were calculated k(1)degrees = 18s(-1) and k(2)degrees = 23s(-1) (Gamma = 2.36 nmol cm(-2)). The modified electrodes were mounted in a flow injection manifold, poised at + 150 mV (vs. Ag/AgCl/3M KCl) and a catalytic current due to the oxidation of NADH was measured. The reproducibility was 1.4% RSD (n = 11 for 30 muM NADH) The behavior of the sensor towards different reducing compounds was investigated. The sensor exhibited good operational and storage stability. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1002/elan.200302902 | - |
| heal.identifier.secondary | <Go to ISI>://000221992100011 | - |
| heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1002/elan.200302902/asset/949_ftp.pdf?v=1&t=h1gmuys2&s=522ed2ef77dff1ec2eaadb6f125723e32125d1dd | - |
| heal.journalName | Electroanalysis | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2004 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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