Ig Mu-Epsilon Isotype Switch in Il-4-Treated Human B-Lymphoblastoid Cells - Evidence for a Sequential Switch
Φόρτωση...
Ημερομηνία
Συγγραφείς
Mills, F. C.
Thyphronitis, G.
Finkelman, F. D.
Max, E. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Immunology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
IgE is produced by B lymphocytes that have undergone a deletional rearrangement of their Ig H chain gene locus, a rearrangement that joins the switch region of the mu-gene, S-mu, with the corresponding region of the epsilon-gene, S-epsilon. To examine the resulting composite S-mu-S-epsilon junctions of human lymphoid cells, we have used a polymerase chain reaction strategy to clone the switch regions of the human myeloma U266 and of two IgE-producing human cell lines generated by treatment of lymphocytes with EBV plus IL-4. The switch junction of one of the EBV lines is a complex rearrangement in which a fragment of S-gamma is interposed between S-mu and S-epsilon. This finding suggested that the switch to epsilon in this human lymphoid cell was preceded by a S-mu-S-gamma recombination. To determine whether this sequential switch rearrangement represented a unique event or occurred with some regularity in human B cells switching to IgE production, DNA samples from bulk cultures of lymphocytes treated with IL-4 were subjected to polymerase chain reaction amplification of their S-mu-S-epsilon junctions. When the resulting fragments were examined by Southern blotting, a substantial fraction hybridized to an S-gamma probe. This finding suggests that sequential recombination involving S-gamma is not rare in the switch to epsilon-production in humans. Our polymerase chain reaction strategy should be useful in studying isotype switching at the DNA level.
Περιγραφή
Λέξεις-κλειδιά
epstein-barr virus, human lymphocytes-b, heavy-chain switch, interferon-gamma, c-epsilon, gene, recombination, mechanism, expression, region
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1992JF47400045
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών