Ig Mu-Epsilon Isotype Switch in Il-4-Treated Human B-Lymphoblastoid Cells - Evidence for a Sequential Switch
| dc.contributor.author | Mills, F. C. | en |
| dc.contributor.author | Thyphronitis, G. | en |
| dc.contributor.author | Finkelman, F. D. | en |
| dc.contributor.author | Max, E. E. | en |
| dc.date.accessioned | 2015-11-24T16:34:46Z | |
| dc.date.available | 2015-11-24T16:34:46Z | |
| dc.identifier.issn | 0022-1767 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7850 | |
| dc.rights | Default Licence | - |
| dc.subject | epstein-barr virus | en |
| dc.subject | human lymphocytes-b | en |
| dc.subject | heavy-chain switch | en |
| dc.subject | interferon-gamma | en |
| dc.subject | c-epsilon | en |
| dc.subject | gene | en |
| dc.subject | recombination | en |
| dc.subject | mechanism | en |
| dc.subject | expression | en |
| dc.subject | region | en |
| dc.title | Ig Mu-Epsilon Isotype Switch in Il-4-Treated Human B-Lymphoblastoid Cells - Evidence for a Sequential Switch | en |
| heal.abstract | IgE is produced by B lymphocytes that have undergone a deletional rearrangement of their Ig H chain gene locus, a rearrangement that joins the switch region of the mu-gene, S-mu, with the corresponding region of the epsilon-gene, S-epsilon. To examine the resulting composite S-mu-S-epsilon junctions of human lymphoid cells, we have used a polymerase chain reaction strategy to clone the switch regions of the human myeloma U266 and of two IgE-producing human cell lines generated by treatment of lymphocytes with EBV plus IL-4. The switch junction of one of the EBV lines is a complex rearrangement in which a fragment of S-gamma is interposed between S-mu and S-epsilon. This finding suggested that the switch to epsilon in this human lymphoid cell was preceded by a S-mu-S-gamma recombination. To determine whether this sequential switch rearrangement represented a unique event or occurred with some regularity in human B cells switching to IgE production, DNA samples from bulk cultures of lymphocytes treated with IL-4 were subjected to polymerase chain reaction amplification of their S-mu-S-epsilon junctions. When the resulting fragments were examined by Southern blotting, a substantial fraction hybridized to an S-gamma probe. This finding suggests that sequential recombination involving S-gamma is not rare in the switch to epsilon-production in humans. Our polymerase chain reaction strategy should be useful in studying isotype switching at the DNA level. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | <Go to ISI>://A1992JF47400045 | - |
| heal.journalName | Journal of Immunology | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1992 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
Αρχεία
Φάκελος/Πακέτο αδειών
1 - 1 of 1
Φόρτωση...
- Ονομα:
- license.txt
- Μέγεθος:
- 1.74 KB
- Μορφότυπο:
- Item-specific license agreed upon to submission
- Περιγραφή: