In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds
Φόρτωση...
Ημερομηνία
Συγγραφείς
Rehder, D.
Pessoa, J. C.
Geraldes, C. F. G. C.
Castro, M. M. C. A.
Kabanos, T.
Kiss, T.
Meier, B.
Micera, G.
Pettersson, L.
Rangel, M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Springer Verlag (Germany)
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Biological Inorganic Chemistry
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
A representative set of vanadium(IV and V) compounds in varying coordination environments has been tested in the concentration range 1 to 10(-6) mM, using transformed mice fibroblasts (cell line SV 3T3), with respect to their short-term cell toxicity (up to 36 hours) and their ability to stimulate glucose uptake by cells. These insulin-mimetic tests have also been carried out with non-transformed human fibroblasts (cell line F-26). The compounds under investigation comprise established insulin-mimetic species such as vanadate ([H2VO4](-)), [VO(acetylacetonate)(2)], [VO2(dipicolinate)](-) and [VO(maltolate)(2)], and new systems and coordination compounds containing OO, ON, OS, NS and ONS donor atom sets. A vitality test assay, measuring the reduction equivalents released in the mitochondrial respiratory chain by intracellular glucose degradation, is introduced and the results are counter-checked with H-3-labelled glucose. Most compounds are toxic at the 1 mM concentration level, and most compounds are essentially non-toxic and about as effective as or more potent than insulin at concentrations of 0.01 mM and below. V-V compounds tend to be less toxic than V-IV compounds, and complexes containing thio functional ligands are somewhat more toxic than others. Generally, ON ligation is superior in insulin-mimetic efficacy to OO or O/NS coordination, irrespective of the vanadium oxidation state. There is, however, no striking correlation between the nature of the ligand systems and the insulin-mimetic potency in these cell culture tests, encompassing 41 vanadium compounds, the results on 22 of which are reported in detail here. The syntheses and characteristics of various new compounds are provided together with selected speciation results. The crystal and molecular structures of {[VO(naph-tris)](2)} [where naph-tris is the Schiff base formed between o-hydroxynaphthaldehyde and tris(hydroxymethyl)amine] are reported. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0311-5 (1).
Περιγραφή
Λέξεις-κλειδιά
vanadium compounds, toxicity, insulin mimesis, cell culture tests, solution speciation, agent bis(maltolato)oxovanadium(iv) bmov, induced diabetic rats, crystal-structure, oxovanadium(iv) complexes, aqueous-solution, v-51 nmr, bioinorganic systems, vanadyl complex, chemistry, speciation
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000174957800004
http://www.springerlink.com/content/0np2g5yfex3wkbtp/fulltext.pdf
http://www.springerlink.com/content/0np2g5yfex3wkbtp/fulltext.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας