In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds

Απλή σελίδα τεκμηρίου
dc.contributor.authorRehder, D.en
dc.contributor.authorPessoa, J. C.en
dc.contributor.authorGeraldes, C. F. G. C.en
dc.contributor.authorCastro, M. M. C. A.en
dc.contributor.authorKabanos, T.en
dc.contributor.authorKiss, T.en
dc.contributor.authorMeier, B.en
dc.contributor.authorMicera, G.en
dc.contributor.authorPettersson, L.en
dc.contributor.authorRangel, M.en
dc.contributor.authorSalifoglou, A.en
dc.contributor.authorTurel, I.en
dc.contributor.authorWang, D. R.en
dc.date.accessioned2015-11-24T16:39:37Z
dc.date.available2015-11-24T16:39:37Z
dc.identifier.issn0949-8257-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8174
dc.rightsDefault Licence-
dc.subjectvanadium compoundsen
dc.subjecttoxicityen
dc.subjectinsulin mimesisen
dc.subjectcell culture testsen
dc.subjectsolution speciationen
dc.subjectagent bis(maltolato)oxovanadium(iv) bmoven
dc.subjectinduced diabetic ratsen
dc.subjectcrystal-structureen
dc.subjectoxovanadium(iv) complexesen
dc.subjectaqueous-solutionen
dc.subjectv-51 nmren
dc.subjectbioinorganic systemsen
dc.subjectvanadyl complexen
dc.subjectchemistryen
dc.subjectspeciationen
dc.titleIn vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compoundsen
heal.abstractA representative set of vanadium(IV and V) compounds in varying coordination environments has been tested in the concentration range 1 to 10(-6) mM, using transformed mice fibroblasts (cell line SV 3T3), with respect to their short-term cell toxicity (up to 36 hours) and their ability to stimulate glucose uptake by cells. These insulin-mimetic tests have also been carried out with non-transformed human fibroblasts (cell line F-26). The compounds under investigation comprise established insulin-mimetic species such as vanadate ([H2VO4](-)), [VO(acetylacetonate)(2)], [VO2(dipicolinate)](-) and [VO(maltolate)(2)], and new systems and coordination compounds containing OO, ON, OS, NS and ONS donor atom sets. A vitality test assay, measuring the reduction equivalents released in the mitochondrial respiratory chain by intracellular glucose degradation, is introduced and the results are counter-checked with H-3-labelled glucose. Most compounds are toxic at the 1 mM concentration level, and most compounds are essentially non-toxic and about as effective as or more potent than insulin at concentrations of 0.01 mM and below. V-V compounds tend to be less toxic than V-IV compounds, and complexes containing thio functional ligands are somewhat more toxic than others. Generally, ON ligation is superior in insulin-mimetic efficacy to OO or O/NS coordination, irrespective of the vanadium oxidation state. There is, however, no striking correlation between the nature of the ligand systems and the insulin-mimetic potency in these cell culture tests, encompassing 41 vanadium compounds, the results on 22 of which are reported in detail here. The syntheses and characteristics of various new compounds are provided together with selected speciation results. The crystal and molecular structures of {[VO(naph-tris)](2)} [where naph-tris is the Schiff base formed between o-hydroxynaphthaldehyde and tris(hydroxymethyl)amine] are reported. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0311-5 (1).en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1007/s00775-001-0311-5-
heal.identifier.secondary<Go to ISI>://000174957800004-
heal.identifier.secondaryhttp://www.springerlink.com/content/0np2g5yfex3wkbtp/fulltext.pdf-
heal.journalNameJournal of Biological Inorganic Chemistryen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2002-
heal.publisherSpringer Verlag (Germany)en
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ