Crystallization of Escherichia coli beta-ketoacyl-ACP synthase III and the use of a dry flash-cooling technique for data collection
| dc.contributor.author | Janson, C. A. | en |
| dc.contributor.author | Konstantinidis, A. K. | en |
| dc.contributor.author | Lonsdale, J. T. | en |
| dc.contributor.author | Qiu, X. | en |
| dc.date.accessioned | 2015-11-24T19:26:05Z | |
| dc.date.available | 2015-11-24T19:26:05Z | |
| dc.identifier.issn | 0907-4449 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/22690 | |
| dc.rights | Default Licence | - |
| dc.subject | 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/*chemistry | en |
| dc.subject | Bacterial Proteins/chemistry | en |
| dc.subject | Crystallization | en |
| dc.subject | Data Interpretation, Statistical | en |
| dc.subject | Escherichia coli/*enzymology | en |
| dc.subject | Freezing | en |
| dc.subject | X-Ray Diffraction/methods | en |
| dc.title | Crystallization of Escherichia coli beta-ketoacyl-ACP synthase III and the use of a dry flash-cooling technique for data collection | en |
| heal.abstract | beta-Ketoacyl-acyl carrier protein (ACP) synthase III (FabH) is a condensing enzyme active in the fatty-acid biosynthesis pathway of bacteria. The enzymes of this pathway provide a set of targets for the discovery of previously unknown antibiotics. FabH from Escherichia coli has been crystallized in two crystal forms using the sitting-drop vapor-diffusion technique. The first form crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 63.1, b = 65.1, c = 166.5 A; the second form crystallized in the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 72.7, c = 99.8 A. A flash-cooling technique using no cryoprotectant was utilized in obtaining data from the second type of crystals. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/10818351 | - |
| heal.journalName | Acta Crystallogr D Biol Crystallogr | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2000 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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