Sequential oligopetide carriers, SOCn, as scaffolds for the reconstitution of antigenic proteins: applications in solid phase immunoassays
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Sakarellos-Daitsiotis, M.
Alexopoulos, C.
Sakarellos, C.
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Elsevier
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peer reviewed
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J Pharm Biomed Anal
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A new class of helicoid type sequential oligopeptide carriers (SOC), for anchoring antigenic epitopes, has been modeled from the repetitive Lys-Aib-Gly (SOCn-I) and Aib-Lys-Aib-Gly (SOCn-II) units aiming to the development of scaffolds with predetermined 3D structures. Conformational analysis showed that the SOCn carriers adopt 3(10)-helical structures, while the SOCn-conjugates retain their original active conformations and they interact neither to the carriers nor to each other. It is concluded that the helicoid structure of SOC,, helps the reconstitution and/or mimicking of the native forms of the epitopes so that potent antigens are generated for developing specific, sensitive and reproducible immunoassays. (C) 2003 Elsevier B.V. All rights reserved.
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oligopeptide carriers, antigenic proteins, solid phase immunoassays, main immunogenic region, nicotinic acetylcholine-receptor, anti-sm antibodies, oligopeptide carriers, glycopeptide dendrimers, synthetic peptide, epitope, autoantibodies, specificity, design
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<Go to ISI>://000220237600005
http://ac.els-cdn.com/S0731708503005612/1-s2.0-S0731708503005612-main.pdf?_tid=ca99cea28e37a9e2463320095358a3e3&acdnat=1333038818_ebd20d32b05b1dd92750c0ef2945b9fe
http://ac.els-cdn.com/S0731708503005612/1-s2.0-S0731708503005612-main.pdf?_tid=ca99cea28e37a9e2463320095358a3e3&acdnat=1333038818_ebd20d32b05b1dd92750c0ef2945b9fe
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας